摘要
目的比较不同实验诊断方法在丙型肝炎(丙肝)抗体检测中的应用价值,并探讨HCV RNA定量与ALT指标之间的关系。方法对105例增强化学发光法(CIA)检测丙肝抗体初筛结果呈阳性(S/Co>1)的标本,分别采用酶联免疫吸附试验(ELISA)和荧光定量聚合酶链反应(RFQ-RT-PCR)进行检测,并对可疑结果(①CIA检测结果S/Co在1～8之间的标本,②ELISA检测结果呈阴性的标本)采用HCV RIBA3.0进行确认;应用OLYMPUS AU-5400全自动生化仪及其生化检测试剂检测全部标本ALT指标。结果ELISA及RFQ-RT-PCR方法检测样本阳性率分别为93.33%和70.48%;对可疑标本经HCV RIBA3.0确认试验显示,2例结果呈阳性(分别为NS3、HCV-C和NS3、NS4阳性),1例为阴性,4例仍为可疑(其中1例HCV RNA>103copies/ml);HCV RNA含量呈阳性的样本中,ALT异常率与HCV RNA含量间呈正相关(r=0.96,P<0.01),而ALT数值的变化与HCV RNA含量并无相关性(r=0.19,P>0.05)。结论第三代ELISA诊断试剂盒检测丙肝抗体同样具有较高的灵敏度,2种检测方法均可能存在假阳性或假阴性情况;RFQ-RT-PCR检测结果阳性率低于CIA与ELISA检测结果;在临床诊断中丙肝抗体的检测应与荧光定量聚合酶链反应一起使用,以提高临床丙肝诊断的准确率。
Objective To evaluate the clinical significance of different methods in detecting HCV antibodies and the relationship among anti-HCV, HCV RNA and ALT. Methods A total of 10^5 samples with positive result （ S/Co〉 1 ） by CIA were detected by ELISA and RFQ-RT-PCR, then HCV RIBA3.0 was used to determine,the samples with negative result by ELISA and those with 1-8 of S/Co by CIA. AU-5400 automated analyzer of Olympus and reagents were used to analyze the ALT of all the samples. Results The positive rate of samples by ELISA and RFQ-RT-PCR were 93.33% and 70.48%, respeetively. In the samples determined by HCV RIBA3.0, 2 were positive in which the HCV bands were NS3, HCV-C and NS3, NS4, respectively, 1 was negative and 4 were uncertain, with HCV RNA quantity higher than 10^3 copies/ml in one of them. The samples with higher quantity of HCV RNA （ more than 10^3copies/ml ） showed higher abnormal rate of ALT （ r=0.96,P〈0.01 ）. However, ALT levels did not correlate with the quantity of HCV RNA （r=0.19,P〉0.05）. Conclusion It is likely to detect false negative and false positive samples by RFQ-RT-PCR and ELISA which is of high sensitivity. As positive detection rate by RFQ-RT-PCR is lower than that by CIA and ELISA, RFQ-RT-PCR should be jointly used in testing HCV antibody so as to improve the accuracy in HCV diagnosis.
出处
《传染病信息》
2007年第5期293-295,共3页
Infectious Disease Information
关键词
增强化学发光法
酶联免疫吸附试验
荧光定量聚合酶链反应
chemiluminescence immunoassay
enzyme-linked immunosorbent assay
fluorescence quantitation-polymerase chain reaction
