摘要
AIM: To establish a high-performance liquid chromatography method for determination of calcium dobesilate in human plasma. METHODS: The chromatographic column was Diamonsil C18 (200 mm×4.6 mm, 5 μm). The mobile phase was composed of acetonitrile: 10 mmol/L ammonium acetate (0.3% triethylamine and pH was adjusted to 3.5 with acetic acid) (7∶93, v/v), at the flow rate of 1.0 mL/min, the column temperature was 40 ℃ and the detector wavelength was set at 305 nm. RESULTS: The calibration curve was linear in the range of 0.36-22.78 μg/mL (r2=0.9998) for determination of calcium dobesilate. The extract recovery was more than 50%. The RSD of inter-day and intra-day were all less than 10%. CONCLUSION: The method is simple, rapid, high efficient, sensitive and suitable for determination of calcium dobesilate in healthy volunteers’ plasma, it can be used in the pharmacokinetics and bioequivalence research of calcium dobesilate.
AIM： To establish a high-performance liquid chromatography method for determination of calcium dobesilate in human plasma. METHODS： The chromatographic column was Diamonsil C18（200 mm × 4.6 mm, 5 μm）. The mobile phase was composed of acetonitrile： 10 mmol/L ammonium acetate （0.3% triethylamine and pH was adjusted to 3.5 with acetic acid） （7 ： 93, v/v）, at the flow rate of 1.0 mL/min, the column temperatm＇e was 40 ℃ and the detector wavelength was set at 305 nm. RESULTS： The calibration curve was linear in the range of 0.36 - 22.78 μg/mL （ r^2 = 0.9998） for determination of calcium dobesilate. The extract recovery was more than 50%. The RSD of inter-day and intra-day were all less than 10%.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2007年第10期1178-1178,共1页
Chinese Journal of Clinical Pharmacology and Therapeutics
