摘要
目的:探讨鲨鱼肝活性肽S-8300对糖尿病小鼠受损胰岛β细胞和肾小球细胞凋亡的影响。方法:用电镜和DNA电泳检测了S-8300对四氧嘧啶(ALX)糖尿病小鼠的胰岛细胞和肾小球细胞凋亡以及测定S-8300对血清NO和一氧化氮合成酶(NOS)水平的影响。结果:电镜检测显示S-8300处理组小鼠可使ALX所致的胰和肾在亚显微结构上的细胞凋亡特征消失,DNA电泳检测表明S-8300处理胰腺和肾组织DNA后梯带消失,能显著降低ALX糖尿病小鼠的血清NO和NOS水平。结论:S-8300的抗ALX损伤与抗凋亡有关,可能是其降血糖作用的机制之一。
AIM：To evaluate the effect of active peptide from shark livers,S-8300,on beta cell of islet and renal glomeruli cells in alloxan induced diabetic mice.METHODS：The anti-apoptotic effect of S-8300 on diabetic mice was investigated by observing pancreas and kidney cells under electron microscope and DNA electrophoresis.The changes of NO and NOS in serum were also been deteced.RESULTS：The ultrastructural apoptosis lesions in pancreas and kidney cells in S-8300 groups were obviously alleviated and significant decrease of NO and NOS in serum was observed in diabetic mice treated with S-8300.CONCLUSION：The hypoglycemic effects of S-8300 was related with its protective activity against the apoptosis.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2007年第9期993-997,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家海洋"863"资助项目(2003A624090)
国家自然科学基金资助项目(30701036)
中国药科大学引进人才启动基金(211032)
