摘要
目的:观察大鼠急性高眼压模型中视网膜一氧化氮合酶(nitric oxidesynthase,NOS)分布及含量的变化,探讨氨基胍(AG)在高眼压诱导的视神经损伤中的神经保护作用。方法:SD大鼠27只,随机选取3只作为空白对照组(6眼),余24只施行高压前房灌注,眼压升高60min后,随机取12只腹膜腔内注射氨基胍溶液(实验Ⅱ组),另外12只腹膜腔内注射等量生理盐水(实验Ⅰ组),再分别按高眼压术后时间不同随机分为4组即12h组、24h组、3天组和7天组,每组3只6眼;各组应用NADPH-d(还原型尼克酰胺嘌呤二核苷酸磷酸-黄递酶)法检测视网膜NOS的表达及分布情况。结果:空白对照组及实验组各组视网膜均有NOS表达,NOS阳性细胞在视网膜主要分布于视网膜节细胞层(ganglion cell layer,GCL)、内丛状层(inner plexiform layer,IPL)、内核层(inner nuclear layer,INL);实验Ⅰ组视网膜GCL的NOS阳性细胞数明显高于空白对照组(P<0.01),实验Ⅱ组视网膜GCL的NOS阳性细胞数与实验Ⅰ组对照减少差异显著(P<0.01)。结论:一氧化氮(NO)参与了高眼压诱导的视神经损伤;氨基胍可能通过对NOS的抑制在大鼠高眼压诱导视网膜损伤中起到视神经保护作用。
Objective：To observe the nitric oxide synthase（NOS） expression in retina, and to investigate the function of the NOS inhibitor aminoguanidine（AG） in rats with acute intraocular hypertension. Methods：Twenty-seven SD rats were randomly divided into three groups：blank control（unoperational group）,experimental group Ⅰ（ocular hypertension group）,experimental group Ⅱ（drug-given group）. Then each experimental group was divided into subgroups at 12 hours,24 hours,3 days and 7 days after perfusion,and NOS expression in the retina were detected by NADPH-diaphorase histochemistry. Results：The staining of NOS were localized in retinas of all groups. The count of NOS staining cells in the experimental group Ⅰ was more significant than that in the blank control. After AG was injected, the levels of NOS decreased. Conclusions：NO should play some cytotoxicity roles to retina. By inhibiting the activity of iNOS,AG might protect the retina against acute ocular hypertension injury.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第9期977-980,共4页
Journal of Nanjing Medical University(Natural Sciences)
