摘要
目的:建立人子宫平滑肌瘤细胞原代培养方法,为子宫肌瘤发病机制及治疗药物筛选的研究提供理想的细胞株系。方法:采用消化法及组织块贴壁2种方法进行子宫平滑肌瘤细胞培养。倒置显微镜下观察原代培养的细胞,并用免疫组化染色对其进行鉴定。结果:2种方法均可获得大量子宫肌瘤细胞,所培养的细胞在倒置显微镜下呈梭形和典型"峰-谷"样生长,α-Actin免疫组化染色呈强阳性。结论:采用该2种方法均可成功进行人子宫平滑肌瘤细胞的原代培养,且培养的人子宫平滑肌细胞生长良好,纯度高,可用于子宫肌瘤发病机制及治疗药物筛选的研究。
Objective：To explore the method of primary culture of uterine leiomyoma cells,which provided a cell line for studying the pathogenesis of uterine leiomyoma and drug screening. Methods：Human uterine leiomyoma cells were cultivated by digestive method and explanted tissue culture in vitro. The surviving cells were observed by phase contrast microscope and identified by immunohistochemistry staining. Results：Many uterine leiomyoma cells were obtained by the above two methods. The cells appeared in spindle shape and showed typical hill-valley pattern under phase contrast microscope. Immunohistochemistry staining with α-Actin demonstrated these cells were positive. Conclusion：Primary culture of uterine leiomyoma cells could be obtained by these two means,with highly purity,and can serve the further studies of the pathogenesis and drug screening of human uterine leiomyoma.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第9期967-969,共3页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京医科大学科技发展基金重点项目(2005NYDZD21)
南京市医学科技发展项目(YKK06080)
关键词
子宫平滑肌瘤
原代培养
免疫组织化学
uterine leiomyoma
primary cell culture
immumohistochemistry
