摘要
目的:分析阿霉素肾病大鼠肾组织结缔组织生长因子(connective tissue growth factor,CTGE)mRNA及蛋白的变化,研究复方鳖甲软肝片的干预怍用及其机制。方法:采用单侧肾切除阿霉素肾病模型。将45只大鼠随机分为5组:正常组、假手术组、模型组、苯那普利组和复方鳖甲软肝片组。用药10周后处死大鼠,观察肾组织病理改变,应用原位杂交和免疫组织化学方法检测肾组织CTGF mRNA及蛋白的表达。结果:正常组及假手术组肾小球系膜细胞、肾小管上皮细胞以及肾间质细胞胞浆内有少量CTGF mRNA及蛋白的黄色颗粒状阳性反应物;模型组上述部位的CTGF mRNA及蛋白的黄色颗粒状反应物呈强阳性表达;复方鳖甲软肝片组CTGF mRNA及蛋白表达较模型组显著减少,与苯那普利组比较,差异无统计学意义。结论:复方鳖甲软肝片可能通过抑制CTGF mRNA及蛋白的过度表达而减缓肾纤维化病程进展。
Objective： To investigate the changes of connective tissue growth factor （CTGF） protein and CTGF mRNA in kidney tissue of rats with adriamycin （ADR）-induced nephropathy and to study the effects of compound Biejia Ruangan tablet （CBJRGT）, a traditional Chinese herbal medicine for treatment of liver fibrosis. Methods. A rat model of ADR-induced nephropathy after one-sided nephrectomy was established. Forty-five Wistar rats were randomly divided into 5 groups： normal control group, sham-operated group, untreated group, Iotensin-treated group and CBJRGT-treated group. Pathological changes of the kidney tissue were observed by microscopy after 10-week drug administration. The expressions of CTGF protein and CTGF mRNA in the kidney tissue were measured by the methods of immunohistochemistry and in situ hybridization. Results： The expressions of CTGF protein and CTGF mRNA in the normal and sham-operated groups were decreased in the intracytoplasm of glomerular mesangial cells, renal tubular epithelial cells and interstitial cells. Compared with the sham-operated group, the expressions of CTGF protein and CTGF mRNA in the untreated group were markedly increased and the development of renal fibrosis in the untreated group could be observed. CBJRGT could significantly decrease the expressions of CTGF protein and CTGF mRNA, and there was no significant difference between CBJRGT-treated group and Iotensin-treated group. Conclusion： CBJRGT may suppress the development of fibrosis through down-regulating the expressions of CTGF protein and CTGF mRNA.
出处
《中西医结合学报》
CAS
2007年第6期651-655,共5页
Journal of Chinese Integrative Medicine
基金
教育部"长江学者和创新团队发展计划"资助项目(No.IRT0413)
国家自然科学基金重点资助项目(No.30130220)
国家中医管理局中医药科研基金(No.2000J-P-31)
关键词
结缔组织生长因子
肾病
鳖甲
大鼠
connective tissue growth factor
nephrosis
carpax trionycis
rats
