摘要
目的:探讨在缺氧/复氧损伤中Na+/H+交换体阻滞剂在不同时相用药的心肌细胞保护作用及其机制。方法:应用乳鼠心肌细胞建立心肌缺血-再灌注(I/R)损伤模型,实验分为对照组、缺氧/复氧全程给药组(EIPA-I组)、复氧前给药1组(EIPA-R1组)和复氧前给药2组(EIPA-R2组)进行缺氧/复氧处理,动态检测单个心肌细胞在缺氧/复氧时细胞胞质Ca2+浓度(即[Ca2+]i)的变化及细胞挛缩程度,并观察细胞的存活率。结果:EIPA-I组和EIPA-R2组显著抑制了[Ca2+]i上升及细胞的挛缩,并提高了细胞存活率。结论:在复氧前给予增大剂量的EIPA与缺氧时常规剂量用药同样有效地抑制复氧期Na+/H+的交换,减轻细胞内钙超载,抑制细胞的挛缩,提高心肌细胞的存活率。
To investigate the protective effects and mechanism of Na^＋/H^＋ exchanger inhibitor on cultured neonatal rat cardiomyocytes during hypoxia/reoxygenation. Methods.. Cultured neonatal rat myocardial cells were divided into control, EIPA-I, EIPA- R1 and EIPA- R2 group. The hypoxia/reoxygenation injury model using the cells was developed. The intracellular free calcium concentrations in single cells were measured with Fura-2/AM, and the cells＇ survival rate was observed simultaneously. Results： In comparison with control group, both EIPA-I and EIPA-R2 group showed a marked decrease in [Ca^2＋ ];and a significant increase in the cells＇ survival rate. However, there was no significant difference between EIPA-R1 and control group. Conclusion： Either a larger dose of EIPA used just before reoxygenation or a general dose added at the beginning of hypoxia can efficiently inhibit Na^＋ /H^＋ exchange and attenuate intracellular Ca^2＋ overload,and reduce the degree of hypercontracture of cardiomyocytes and enhanced the survival rate of myocytes.
出处
《武汉大学学报（医学版）》
CAS
2007年第6期698-702,共5页
Medical Journal of Wuhan University
关键词
钙超载
钠氢交换体抑制剂
缺氧/复氧损伤
乳鼠心肌细胞
Calcium Overload
Na^＋/H^＋ Exchanger Inhibitor
Hypoxia/reoxygenation Injury
Neonatal Rat Cardiomyocytes
