摘要
Myosin subfragment-1 was prepared from the myofibrils of bighead carp (Aristichthys nobilis). The myosin subfrag- ment-1 was proved to have the activity of tripolyphosphatase (TPPase) responding to the hydrolysis of sodium tripolyphosphate (STPP). The optimum temperature and pH for the TPPase of myosin subfragment-1 were 30℃ and pH 5.0, and at pH 8.0 the TPPase also showed a high activity. Mg2+ was necessary to TPPase. The TPPase activity of myosin subfragment-1 was activated by Mg2+ under low concentrations, but was inhibited when the concentration was over 17 mmolL-1. The TPPase activity was also affected by KCl. The optimum concentration of KCl for TPPase was 0.3 molL-1 under the condition of 17 mmolL-1 Mg2+. The TPPase activity was significantly inhibited by EDTA-Na2. Reagents such as KBr, KI and KIO3 could inhibit the TPPase effectively. K2Cr2O7 as well as KMnO7 and KNO3 exhibited weak inhibiting effects. The TPPase converted STPP to pyrophosphate (PP) and orthophosphate (Pi) stoichiometrically with a KM of 3.2 mmolL-1.
Myosin subfragment-1 was prepared from the myofibrils of bighead carp （Aristichthys nobilis）. The myosin subfragment-1 was proved to have the activity of tripolyphosphatase （TPPase） responding to the hydrolysis of sodium tripolyphosphate （STPP）. The optimum temperature and pH for the TPPase of myosin subfragment-1 were 30℃ and pH 5.0, and at pH 8.0 the TPPase also showed a high activity. Mg^2＋ was necessary to TPPase. The TPPase activity of myosin subfragment-1 was activated by Mg^2＋ under low concentrations, but was inhibited when the concentration was over 17 mmolL^-1. The TPPase activity was also affected by KCl. The optimum concentration of KCl for TPPase was 0.3 molL^-1 under the condition of 17 mmolL^-1 Mg^2＋. The TPPase activity was significantly inhibited by EDTA-Na2. Reagents such as KBr, KI and KIO3 could inhibit the TPPase effectively. K2Cr2O7 as well as KMnO7 and KNO3 exhibited weak inhibiting effects. The TPPase converted STPP to pyrophosphate （PP） and orthophosphate （Pi） stoichiometrically with a KM of 3.2 mmolL^-1.
基金
This work was supported by the National Natural Science Foundation of China （No. 30671632）
supported by the National High-tech Research and Development Project of China （No. 2006AA09Z444）.
