摘要
目的初步探讨口腔鳞状细胞癌中凋亡抑制蛋白survivin的表达和临床意义,以及survivin的表达与bcl-2,P53,bax表达之间的关系。方法采用免疫组化二步法,DAB染色。27例口腔鳞癌和1例乳腺癌阳性对照病例手术前均未进行放疗、化疗或生物治疗。结果27例口腔鳞癌组织中survivin阳性表达率为88.89%(24/27),11例正常组织中survivin没有表达。在口腔鳞癌不同的临床病理分级survivin的表达有差异(P<0.05),其表达随着临床病理分级的增加而增加。Survivin在有淋巴结转移者阳性表达率(100%)高于无淋巴结转移者(82.35%),但统计学比较无差异(P>0.05)。口腔鳞癌中survivin的表达在不同的年龄组和性别间无差异(P>0.05),而在不同部位的鳞癌中表达有差异(P<0.05)。口腔鳞癌中survivin的表达和bcl-2,突变型P53的表达成正相关,(P<0.05),而与bax的表达成负相关,(P<0.05)。结论①Survivin可作为口腔鳞癌诊断的一项重要指标;②Survivin可作为口腔鳞癌基因治疗和免疫治疗的靶分子;③在口腔鳞癌基因治疗方面可以考虑协同抑制突变型P53和bcl-2的表达,或者提高bax的表达来提高疗效。
Objective To examine the expression of survivin, a novel member of inhibitors of apoptosis protein （IAP） in oral squamous cell carcinomas （OSCC） and the relationship between the expression of survivin and that of Bcl-2,p53,Bax.Methods The expressions of surviving, Bcl-2, p53, and Bax in 27 cases of OSCC and 1 case of mammary carcinoma and 11 cases of normal oral tissues were examined by immunohistochemistry. All the patients were not treated with chemotherapy, radiotherapy and biologic therapy before operation.Results The expression of survivin was observed in 88.8% （24/27） OSCC samples and none in normal oral tissues. The expression of survivin was increased with high pathologic grades. Survivin expression was not associated with lymph node metastasis, age, and sex （P〉0.05）. The expression of survivin was correlated with the expression of Bcl-2 and p53, but negatively related with the expression of bax （P〈0.05）.Conclusion The expression of survivin is correlated with the malignant potential of OSCC. Survivin may cooperate with Bcl-2 and antagonize p53 and Bax.
出处
《现代口腔医学杂志》
CAS
CSCD
北大核心
2007年第4期410-413,共4页
Journal of Modern Stomatology
