摘要
应用酶联免疫吸附试验（ＥＬＩＳＡ）对贵州省主要家畜大肠杆菌不耐热肠毒素（ＬＴ）进行了研究、同时与平板免疫溶血试验（ＰＩＨＡ）、基因探针（Ｇｅｎｅｐｒｏｂｅ）测定ＬＴ进行了比较。通过对贵州省主要地区的猪、鸡、牛等２３９份腹泻样品中的１２４份Ｅ．ｃｏｌｉ分离物测定，ＬＴ阳性检出率为ＥＬＩＳＡ５１．６％、Ｇｅｎｅｐｒｏｂｅ６１．３％、ＰＩＨＡ１３．５％．结果表明，肠毒素大肠杆菌是引起家畜腹泻致病的主要原因，尤其是合肥地区检出率更高。
Heat - Labile Enterotoxin (LT) of enterotoxigenie E coli (ETEC) from animal origins in Guizhou was detected by Enzyme -Linked immunosorbant assay (ELISA ). Detection of LT of E. coli from 124 fecal specimens were conducted by three methods. 64,51 and 76 samples were determinded to be ETEC-petive fecal specimens by ELISA, Plate immune haemolysis assay (PIHA) and Gene probe respecively. The results showed that Enterotoxigenic Escherichia coli was a important agent that caused diarrhoea in animals.
基金
国家自然科学基金
