摘要
运用HPLC测定四川境内乌头中3种酯型生物碱-新乌头碱、鸟头碱和次乌头碱的含量．实验以C8色谱柱为固定相，为乙腈-甲醇-缓冲液（8：39：53），缓冲液为0．2％冰醋酸，用二乙胺调pH值至5．41；流速为1．0mL／min；紫外检测波长为231nm．所得新乌头碱、次乌头碱和乌头碱的标准曲线范围分别是11．968-299．2μg／mL、5．552-138．8μg／mL和7．168～179．2μg／mL（r=0．999 52,0.999 57,0.999 56,n=7；检测限分别是0．4787μg／mL、0．2776μg／mL和0．7168μg／mL加样回收率为95．36％～101．77％，RSD均小于5％．结论表明该方法准确、灵敏度高，便于乌头类药材质量控制．
The contents of mesaconitine, hypaconitine and aconitine in Aconitum carmichaeli Debx. in Sichuan were determined by HPLC. Samples were analyzed on an Alltech Apollo C8 Column, the mobile phase was acetonitrile-methanol-buffer solution （8： 39 ： 53 v/v）, and the buffer solution was pH 5.41, 0.2% （v/v） acetic acid and diethyl amine. The flow-rate was kept at 1.0 mL/min while the detective wavelength was 231nm. The results showed that the calibration curves for mesaconitine, hypaconitine and aconitine were linear within the concentration range of 11. 968-299.2 μg/mL, 5. 552-138.8 μg/mL and 7. 168-179.2 μg/mL （r=0. 999 52, 0. 999 57 and 0. 999 56, n=7）, respectively. Their limit of detection was 0. 478 7μg/mL, 0. 277 6 μg/mL and 0. 716 8 μg/mL, respectively. The average recovery was within 95.36%-101.77%. RSD were all below 5%. It is conclused that the method is accurate, highly sensitive and suitable to quality control of A. carmichaeli Debx.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第10期61-66,共6页
Journal of Southwest University(Natural Science Edition)
基金
四川省科技厅应用基础研究资助项目(04JY029-005-4)
四川省教育厅重点资助项目(2004A010)
关键词
高效液相色谱法
乌头
鸟头碱
新鸟头碱
次乌头碱
High-performance liquid chromatography
Aconitum carmichaeli Debx.
Aconitine
Mesacontine
Hypaconitine
