摘要
目的观察体外尿毒症血清环境诱导的脐静脉内皮细胞(HUVEC)活性氧(ROS)及白介素-6(IL-6)的产生及左旋肉毒碱(L-CN)对它们的影响。方法以2、7-二氢二氯荧光素染色(DCFH)和荧光分光光度计法检测ROS强度,RT-PCR和ELISA方法检测IL-6表达,观察尿毒症血清对HUVEC的影响和不同浓度L-CN(25μM,250μM,1000μM,2500μM)的干预作用。结果尿毒症血清环境能使细胞内ROS产生及IL-6表达明显增加;而L-CN可明显抑制尿毒症血清环境中细胞内ROS产生及IL-6表达,且有浓度依赖效应。结论尿毒症血清促使血管内皮微炎症状态的建立,L-CN可抑制其组织活性氧及白介素-6的产生,减轻尿毒症患者慢性炎症反应,对血管粥样硬化病变可能有延缓作用。
Objective To observe the effect of uremic serum on the production of reactive oxygen species （ROS） and interleukin-6 （IL-6） in human umbilical vein endothelial cells （HUVEC）, and its intervention by L-carnitine （L-CN）. Methods Uremic sera mixed with various concentrations of L-CN （0, 25, 250, 1000 and 2500μM） were added to the culture medium. Semi-quantitative RT-PCR and ELISA were applied to evaluate the expression of IL-6. Cells labeled with oxidation-susceptible fluorescent probe 2,7-dichorofluoresin diacetate （DCFH） and measured by spectrofluorophotometer were used for ROS assay. Results IL-6 and ROS were increased in HUVEC in the presence of uremic serum. With the addition of L-CN （250μM-2500μM） in culture medium, the production of ROS and IL- 6 in HUVEC was inhibited, and the inhibition was L-CN concentration dependent. Conclusion Uremic serum induces the micro-inflammation processes of blood vessel endothelia. L-CN inhibits the production of ROS and IL-6 in HUVEC and thus the microinflammation and atherosclerosis processes in uremic patients.
出处
《中国血液净化》
2007年第10期534-537,共4页
Chinese Journal of Blood Purification
