摘要
应用MS＋2．0mg·L(-1)2，4-D＋0．5mg·:L(-1)BA作为培养基，附加500mg·L(-1)水解酪蛋白和w=3％蔗糖，可以从玉米叶片愈伤组织中直接诱导出体细胞胚胎．观察表明，玉米胚性细胞同非胚性细胞差别显著．玉米幼叶体细胞胚胎可能起源于单细胞；发育早期的胚性细胞团与周围薄壁细胞存在着明显的界限，推测可能同体细胞胚胎发生初期的生理隔离有关，它随着胚胎发育而逐渐消失．玉米体细胞胚胎胚柄发达，由一列或多列细胞组成．玉米叶片体细胞胚胎主要发生于愈伤组织的表皮层或表皮层下部．
Somatic embryos could be induced directlyfrom the most basalsegments of the inneryoung leaves of maize seedling (12-15d, 15-20cm tall) on the MS medium with 2.0 mg' L-12, 4-D and 0.5mg' L-1 BA, adding 500mg' L-1 CH and w=3% sucrose. Histological evidenCeshowed that embryonic cell was very different from non-embryonic cell of maize in cytology.Somatic embryos from maize leaf explants might be of single cell There. were distinct bordersbetween the small cell groups of embryonic cells and parenchyma cells around them It wasinferredthat they mightbe in relation to phytiological isolation at early development stage of somatic embryos. This borders were disappearinggradually with embryoid development The suspensor ofsomatic embryos embryos inmaize was very developedwith one or a few lines of cells. somaticembryogenesis of young leaf of maizewas from the epidermalor subepidermal layers of callus.
出处
《北京师范大学学报（自然科学版）》
CAS
CSCD
北大核心
1997年第1期112-116,共5页
Journal of Beijing Normal University(Natural Science)
