摘要
目的:建立高效毛细管电泳法同时测定消炎抗菌片中黄芩苷和没食子酸的含量。方法:定量采用内标法,选取苯甲酸钠为内标物。所用毛细管规格为60 cm×75μm(有效长度为45 cm),检测波长为215 nm,电压为21 kV,运行缓冲液:10 mmol·L^(-1)硼砂缓冲液(pH为9.0),柱温为20℃,压力进样(8s)。结果:黄芩苷的线性范围为48～576μg·ml^(-1)(r= 0.9997),加样回收率为98.3%,RSD为3.0%;没食子酸的线性范围为34～204μg·ml^(-1)(r=0.9992),加样回收率为99.0%,RSD为3.1%。结论:高效毛细管电泳法同时测定消炎抗菌片中黄芩苷和没食子酸的含量是可行的。本方法简便、快速、准确、重复性好。
Objective： To determine the contents of baicalin and gallic acid in Xiaoyankangjun Tablets by HPCE. Method： The fused-silica capillary （60 cm×75 μm）（ effective length 45 cm） was used, the detection wavelength was 215 nm and applied voltage was 21 kV, the 10 mmol·L^-1 borate buffer solution （ pH 9.0） was used as running buffer solution, the capilary column temperaruve was 20 ℃. The internal standard method was used, the sodium benzoate as internal standard. The sample was injected by pressure（8s）. Result： The linear ranges of the baicalin and gallic acid were at 48 -576 μg·ml^-1 （r = 0.999 7）and 34 -204 μg·ml^-1 （r = 0.999 2） ,respectively, the average recoveries （n = 6） were 98.3% and 99.0% with RSD 3.0% and 3.1% , respectively. Conclusion： The method is simple, rapid and accurate, it can be used for quality control of Xiaoyankangjun Tablets.
出处
《中国药师》
CAS
2007年第11期1069-1071,共3页
China Pharmacist
