摘要
本文运用气管环血清中和试验对１２个ＩＢＶ毒株进行了血清型研究。以气管环纤毛运动为指示系统，以能中和２．０ｌｏｇ１０ＣＤ５０同源病毒血清效价为１个抗体单位，含２０个抗体单位的血清与等量病毒作用测定血清对纤毛运动保护百分率，以欧氏距离数字分类分型，并用ＳＰＳＳ软件聚类分型分析。结果表明ＳＡＩＢ１属Ｍ４１型，ＳＡＩＢ３属Ｔ型；ＸＪＩＢ接近Ｍ４１型，ＳＨＩＢ、ＳＡＩＢ２接近Ｔ型。ＸＪＩＢ，ＳＨＩＢ，ＳＡＩＢ２具有过渡性（变异性）特征。
Neutralisation test in tracheal organ cultures was employed to make a study on the serotype of 12 IBV strains in this paper,using ciliary activity of tracheal organ cultures as indicator system.One antibody unit was defined as the serum dilution which neutralised an exact 2.0log 10 CD 50 of homolgous virus.After antiserum containing 20Ab units mixed with virus to determine the percentage of reciprocal neutralisation,Euclidean distance were calculated,and numerical taxonomy was performed.On the basis of Euclidean distance,computer program(SPSS)was utilised to make dendrogram.The results showed that SAIB 1,SAIB 3 belonged to M 41 type and T type respectively;XJIB was close to M 41 type,while SHIB,SAIB 2 were close to T type.XJIB,SHIB,SAIB 2 showed cross reactions and variable character.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1997年第4期366-371,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
传染性支气管炎
病毒
血清学分型
鸡病
Avian infectious bronchitis virus,Tracheal organ culture serum neutrlisation test,Numerical taxonomy,SPSS cluster classfcation
