摘要
检测１５头奶牛离体外周血单核细胞（ＰＢＭＣ）和体内产生抗体免疫应答的结果表明，１．０μｇ／ｍｌ的ＰＷＭ或ＳＥＢ使ＰＢＭＣ增殖达最高值，但在分泌Ｉｇ中却表现出不同作用；还发现在群体内离体细胞增殖强度的变异程度（Ｃ．Ｖ≤１２．５％）小于分泌的Ｉｇ量的变异程度（Ｃ．Ｖ＝３１．９％～６２．２％，其中个别Ｉｇ量超出ｘ±２ＳＤ）。１５头奶牛在实验期间（１８０ｄ）的１２次血清样品中ＩｇＭ、ＩｇＧ１和ＩｇＧ２水平变异程度（Ｃ．Ｖ≤１０％）也小于它们个体之间的变异程度（Ｃ．Ｖ＝１１．８～１５．８％）。在对卵清蛋白为载体的异硫氰酸荧光素（ＦＩＴＣ）的特异性反应中，初次和再次反应的抗体滴度（ＩｇＧ１和ＩｇＧ２）呈明显相关（ｒ＝０．６９８和０．８６７，ｎ＝１５；Ｐ＜０．０１）；而个体之间抗体滴度的变异程度大（Ｃ．Ｖ＝２８．４％～３６．７％），个别超出ｘ±２ＳＤ。分析表明，在ＰＢＭＣ分泌Ｉｇ水平或抗体反应滴度变异的个体与ＰＢＭＣ增殖强度和血清Ｉｇ水平无关。这些说明在免疫功能正常并相近的同种动物群体内，仍存在一定数量的个体。
This page reports the study of the immune response of boPBMC in vitro to mitogens in the proliferation or Ig production and of cattle in vivo to FITC with ovalbumin as carrier at the same time.PWM or SEB,which induced boPBMC strongly to proliferate in the concentration of 1.0μg/ml,could induce cells to produce Ig or not.It is found that there was smaller different extent among the tested cattle in the intensity of cell proliferation(C.V≤12.5%,n=15) than in the production of Ig(C.V≥31.9%,even 62.2%) by PWM or SEB (0.01～1μg/ml) induced PBMC, in some individual the produced Ig would exceed x±2SD.The level of Ig classes and IgG subclasses in the serum kept relative steady not only in the individual (C.V≤10%,n=12),but also in the group of these animals (C.V=11.8～15.8%,n=15) in the tested period about 180 days.Meanwhile,after immunization of FITC ovalbumin,the titers of anti FITC antibody (including their Ig classes)in sera from these animals showed significant difference (C.V≥28.4%～36.7%),even some exceeded x±2SD,(p<0.05).In addition,there was a linear regression between the antibody titers in the primary and secondary reactions (r=0.698,IgG 1;0.867,IgG 2,n=15,p<0.01);nevertheless no regression could be found between the antibody titers in vivo or the produced Ig in vitro and Ig levels in sera or cell proliferation in vitro. All these results suggest that the homogeneous population,which have normal immune function,could show significant difference in the response to an identical antigen or mitogen because of the immune response gene.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1997年第4期289-294,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
