摘要
目的从海藻中分离海藻真菌并通过抗肿瘤活性筛选获得活性菌株。方法从样品中选择性分离得到真菌,并采用海虾生物致死法和人体慢性髓性白血病细胞K562为筛选模型对所得真菌的发酵产物进行抗肿瘤活性筛选。结果从黄海海岸潮间带采集到的28种海藻中分得221株真菌,经筛选得到具有海虾生物致死及细胞毒活性菌株各8株。结论实验结果表明,海藻真菌是寻找抗肿瘤活性物质的重要资源。
Objective To isolate algicolous fungi from algae and obtain antitumor strains. Methods Selective isolation of algicolous fungi from algae was done, and antitumor activity corresponds to their lethiferous effect on brine shrimp and cytotoxicity against K562 cell was determined. Results 221 strains of algicolous fungi were isolated from 28 algae samples collected from intertidal zones of the Yellow Sea. Eight strains showed lethiferous activity and eight showed cytotoxicity. Conclusion The results indicated that algicolous fungi would be one of the important resources to search for new antitumor compounds.
出处
《中国海洋药物》
CAS
CSCD
2007年第5期1-5,共5页
Chinese Journal of Marine Drugs
基金
国家863高科技发展计划(No.2007AA09Z447)
关键词
海藻真菌
分离
抗肿瘤活性
筛选
algicolous fungi
isolation
antitumor activity
screening
