摘要
通过RT-PCR特并性扩增出新城疫病毒（NDV）山东分离株（ShD-5-04）的F基因序列，对其进行核苷酸序列测定和分析。结果表明，ShD-5-04株的F基因开放性阅读框为1662bp，编码489个氨基酸。与国外发表的部分新城疫病毒强毒株和弱毒株之间相同序列进行比较，F基因核苷酸序列的同源性在84．1％～88．7％之间，氨基酸同源性在88．1％～93．3％之间；F蛋白裂解位点区（112位～117位）氨基酸组成与强毒株一致，从基因水平上说明NDVShD-5-04株为新城疫强毒株。
One atypical newcastle disease virus（NDV） isolated from a chicken farm in Shandong Weifang in the year 2004 that without any typical ND symptom was studied. This test amplified Fusion （F） gene of Shandong isolate strain （ShD-5-04） by reverse transcription-polymerase chain reaction （RT-PCR） tech- nique. The F gene was sequenced after the products of amplification being cloned. The results showed that：the F gene of Shandong isolate strain （ShD-5-04） has 1 662 bp length in opening reading fragment （ORF） and encods 489 amino acids. Compared with the published virulent strains and avirulent strains of NDV, the homology of the nucleotide of F gene is 84.1%-88.7% and the homology of the deduced amino acid sequences of F gene is 88.1%-93.3% ;The amino acid sequences of cleavage site regeion （112-117） of F gene were similar to that of all other virulent strains. The results also showed that the Shandong isolate strain （ShD-5-04） was a virulent strain of DNV. It can concluded that antibody impression is the reason to cause atypical ND in this case, not caused by variant virus.
出处
《动物医学进展》
CSCD
2007年第10期1-7,共7页
Progress In Veterinary Medicine
基金
山东省财政支持项目(SDGP2004-54-O)
关键词
新城疫病毒
分子生物学
非典型新城疫
鸡
Newcastle disease virus
molecular biology
atypical newcastle disease
chicken
