摘要
目的:采用MTT法评价中药制剂金栀含漱液、氢氧化钙药尖及FC对人牙周膜成纤维细胞(HPDLF)的细胞毒性及毒性的可逆性。方法:体外培养HPDLF,选用金栀含漱液作为实验组(A组),氢氧化钙药尖浸提液为阴性对照组(B组),FC(C组)为阳性对照组,培养基作为空白对照组,分别与HPDLF接触1、3、5、7d时用MTT法测细胞增殖率及接触第1天的回复度。结果:A组及B组对HPDLF增殖无显著性抑制作用,细胞毒性可逆;C组对HPDLF增殖有显著的抑制作用,细胞毒性不可逆。FC组与金栀含漱液组和氢氧化钙药尖组相比抑制作用均有显著性差异,后两组的作用与培养基相当。结论:中药制剂金栀含漱液对HPDLF无明显细胞毒性作用。
AIM ： To evaluate the cytotoxicity of traditional Chinese medicine jinzhi gargle, calcium hydroxide points and FC on the human periodontal ligament fibroblasts. METHODS： Human periodontal ligament fibroblasts （HPDLF） were cultured in vitro with three intracanal medications mentioned above. The MTT assay was employed to evaluated the cytotoxic action. RESULTS： Jinzhi gargle and calcium hydroxide points did not inhibit the proliferation of HPDLF and tend to be lower toxicity with the concentrations decreasing and the culture time increasing,while FC showed higher toxicity. CONCLUSION： The jinzhi gargle showed no detectable cytotoxicity on the HPDLF.
出处
《牙体牙髓牙周病学杂志》
CAS
2007年第10期576-578,共3页
Chinese Journal of Conservative Dentistry
基金
四川省科技厅重点攻关项目资助(05SG022-019-10)
关键词
金栀含漱液
细胞毒性
人牙周膜成纤维细胞
jinzhi gargle
cytotoxicity
human periodontal ligament fibroblasts
