摘要
对5种野生芍药和26个栽培芍药品种的亲缘关系进行了RAPD分析。17条引物在所有供试样本中共扩增出153条反应带,其中多态带149条。对RAPD扩增结果采用UPGMA法进行聚类分析,在遗传相似度0.32处,块根芍药(Paeonia anomala)被单独分为一类,其它30份材料归为另一类;在遗传相似度0.54处,川赤芍(P.veitchii)、草芍药(P.obovata)和美丽芍药(P.mairei)3个野生种先后从30份材料中分别单独分离出来,而野生种芍药(P.lactiflora)则与所有的栽培品种聚为一类。该结果表明26个品种间的遗传背景相对一致,并与野生种芍药的遗传基础最为近密,与其它4种野生芍药的遗传差异则较大。按照与栽培品种亲缘关系由近到远的次序,4个野生近缘种依次排布为:美丽芍药、草芍药、川赤芍和块根芍药。研究结果从分子水平揭示出中国芍药品种群遗传背景狭窄,利用野生近缘种改良现有品种尚具有广阔空间。
Relationship among 5 wild species and 26 cultivars were analyzed using RAPD（Random am- plified polymorphic DNA）markers. One hundred and fifty three amplified fragments were detected by applying 17 10-mer primers, among them 149 fragments were polymorphic. Cluster analysis was then performed by UPGMA method. In the dendrogram based on the genetic similarity coefficients, Paeonia anomala were separated from other 30 samples at GSC（Genetic Similarity Coefficient）of 0. 32; And at GSC of 0. 54, P. veitchii, P. obovata and P. mairei were individually separated, while P. lactiflora was still linked together with all of the cuhivars. The result suggested high genetic homogeneity between the cultivars and P. lactiflora. Comparatively, the other 4 wild species showed distant genetic relationship with the cultivars, and the order based on the GSC from close to distant was arranged as P. mairei, P. obovata, P. veitchii and P. anomola. This molecular study revealed the narrow genetic background of the cultivated peonies native to China, so there exists broad space of improving the cultivars with wild species.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第5期1321-1326,共6页
Acta Horticulturae Sinica
基金
国家林业局重点基金项目(97-17)
山东农业大学中青年创新基金项目(23214)
