摘要
采用AFLP分子标记技术对47种苹果属植物进行遗传多样性和亲缘关系分析,其中24个为国外引进的观赏海棠品种。结果表明,筛选出的10对分辨率强的引物共扩增出589条带,其中多态性带534条,多态位点百分率达90.66%。观赏海棠品种在DNA分子水平上表现出极为丰富的遗传多样性。基于Jaccard系数对AFLP扩增结果进行UPGMA(Unweighted Pair-Group Method with Arithmetic Mean)聚类,以相似系数0.60为标准,将供试材料分为8组,以相似系数0.59为标准,将观赏海棠品种分为4组。分析认为,供试的24个观赏海棠品种中有22个品种与我国原产的苹果属植物亲缘关系较近。
In this study, 24 crabapple cultivars and 23 wild species of Malus were analyzed using AFLP （Amplified Fragment Length Polymorphism）molecular marker in order to test the genetic diversity and relationship of the materials. The results showed that a total of 589 informative AFLP fragments were generated with 10 EcoR I-Mse I primer combinations, of which 534 ones were polymorphic and the polymorphism rate was 90. 66%. Rich genetic diversity of crabapple germplasm resources was detected at DNA level. UPGMA （Unweighted Pair-Group Method with Arithmetic Mean）cluster analysis based on Jaccard coefficient indicated that the materials used in the study could be divided into 8 groups, crabapple cultivars were divided into 4 groups. In the present study, 22 of 24 cultivars tested were closely related with species of Malus in China.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第5期1227-1234,共8页
Acta Horticulturae Sinica
基金
北京市教委科技计划重点项目
北京市自然科学基金项目(KZ200410020009)
北京市科技新星计划人才培养项目(2006B25)
关键词
观赏海棠
亲缘关系
遗传多样性
AFLP
Malus
Crabapple
Phylogenetic relationship
Genetic diversity
AFLP
