摘要
以盆栽5年生云锦杜鹃苗木为材料,分别设对照、轻度胁迫、中度胁迫、重度胁迫4个处理,研究土壤水分胁迫对云锦杜鹃叶片脂类过氧化及抗氧化系统的影响。结果表明:与对照和轻度胁迫相比,中度和重度胁迫导致了活性氧产生速率、丙二醛含量、脯氨酸含量、质膜透性和过氧化物酶活性显著升高;谷胱甘肽含量、类胡萝卜素含量和过氧化氢酶活性显著降低。而抗坏血酸含量、超氧化物歧化酶活性和抗坏血酸过氧化物酶活性则在中度水分胁迫下上升,重度水分胁迫下下降。过度水分胁迫导致脂类过氧化严重,对云锦杜鹃生长造成一定的伤害。
Rhododendron fortunei is one of the famous ornamental flowers in China, but its water conditions for growth are less known. Effects of soil water stress on antioxidant system and lipid peroxidation in the leaves of R.fortunei were investigated by using 5-year-old pot-cultured seedlings as experimental materials and exposing them to different levels of water stress. Twelve pots of plant seedlings with a similar growth were divided into 4 groups： no stress（control）, light stress, moderate stress and heavy stress. In comparison with no stress and light water stress, the moderate stress and heavy stress resulted in significant increases in the malondialdehyde（MDA）content, generating rate of superoxide anion（O2）,proline content, peroxidase（POD） activity and membrane permeability of leaf cells and significant decreases in glutathione（GSH）contents, carotenoid content and catalase（CAT）activity. However, the content of ascorbic acid（AsA）,activities of superoxide dismutase（SOD）and ascorbate peroxidase（APX）exhibited another trend, i.g. increased under moderate water stress, but declined under heavy water stress. The present conclusion is that R. fortunei growth can be inhibited through enhancing lipid peroxidation under heavy water stress.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第5期1217-1222,共6页
Acta Horticulturae Sinica
基金
浙江省自然科学基金项目(Y504256)
关键词
云锦杜鹃
水分胁迫
脂类过氧化
抗氧化系统
Rhododendron fortunei
Water stress
Lipid peroxidation
Antioxidant system
