摘要
以甜瓜雌雄异花同株和雄全同株近等基因系为材料,采用高效液相色谱(HPLC)技术,分析雌花、雄花和两性花4种内源激素和4种多胺含量的动态变化。结果显示,雌花和两性花发育初期和后期GA_3含量高于雄花;多数时期雄花ZT和IAA含量高于雌花和两性花;雄花发育中、后期ABA含量较大幅度上升,且明显高于雌花,两性花ABA含量变化趋势与雌花相近,但整体明显高于雌花;ZT/GA_3比值雄花高于雌花和两性花。由此表明,较高的GA_3含量,较低的ZT和IAA含量有利于雌蕊的发育,较高的ABA含量有利于雄蕊中、后期的发育。花蕾发育初期,尸胺(Cad)含量从高到低依次为雌花、两性花和雄花,随着发育的进行,雄花和两性花中Cad含量呈现较大幅度上升,而雌花始终保持在相对较低的水平。因此,相对较高的Cad含量可能有利于早期雌蕊的发育,较低的Cad含量有利于雄蕊的发育,而后期则相反,高含量的Cad有利于雄蕊的成熟;高含量的Spd可能有利于早期雌蕊的发育。
The female, male and hermaphrodite flowers in different development stages of monoecious and andromonoecious near-isogenic lines of melon were used as materials to test the contents of 4 internal hormones and 4 polyamines with HPLC. The results showed that GA3 content was higher in the young and old female and hermaphrodite flowers than in the male flowers; ZT and IAA content was higher in male flowers than in the female and hermaphrodite flowers for most of time in development period. ABA content rose obviously in middle and late development period of male flowers, and was higher than female flowers. The change trend of ABA content in hermaphrodite flowers was similar but ABA content markedly higher than female flowers; ZT/ GA3 in male flowers were higher than the female and hermaphrodite flowers. Thus it could be considered that higher GA3 content, lower ZT and IAA content were beneficial to development of pistil, and higher ABA content was beneficial to development of stamen in middle and late development period. The order of Cad content was female, hermaphrodite and male flowers from high to low in the young period of flower bud. Cad content rose obviously in male and hermaphrodite flowers along with the development of flower bud, but it always was lower in female flowers. So higher Cad content was beneficial to development of pistil and lower content was beneficial to development of stamen in the early stage. On the contrary, higher Cad content promoted ripeness of stamen. Higher Spd content was beneficial to development of pistil in the early stage.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第5期1195-1200,共6页
Acta Horticulturae Sinica
基金
甘肃省农牧厅农业生物技术专项(GNSW-2006-03)
