摘要
以黄瓜纯合自交系9110Gt(bibi)和03828(BiBi)为双亲获得的F_2代为试材,利用分离群体组群分析法,从256对AFLP选择性引物中获得了与营养器官无苦昧位点连锁距离为6.43 cM的AFLP分子标记TG/GCT_(150),并成功地将其转化为操作简便、表现稳定的SCAR标记SC_(87)。
In this study, AFLP technique and the method of bulked segregant analysis were used to study the F2 progeny of purified cucumber line 9110Gt（bibi）×03828（BiBi）.One AFLP molecular markers TG/GCT（150）linked to non-bitterness gene in cucumber with a genetic distance 6.43 cM were identified from 256 primer combinations. This band was collected and sequenced to synthesize a sequence characterized amplified region（SCAR）primer, and TG/GCT（150）was converted to SCAR marker SC（87）successfully.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第5期1177-1182,共6页
Acta Horticulturae Sinica
基金
国家‘863’项目(2006AA10Z1A8)
农业部蔬菜遗传与生理重点开放实验室资助项目
