摘要
为研究不同柑橘类型对柑橘衰退病病毒构成的影响,将9个保存在新会橙上的引起甜橙茎陷点症状的柑橘衰退病毒强毒分离株嫁接接种到凤凰柚后,比较了接种前后p25基因的Hinf I RFLP和SSCP变化情况。分离株CT90与CT20接种前后的RFLP组群没有发生变化;RFLP组群发生变化的分离株中有6个由混合组群变为单一组群。此外,除分离株CT90接种前后的SSCP谱带保持不变外,其余8个分离株从新会橙接种到凤凰柚后,SSCP谱带数均呈减少趋势。试验结果倾向于表明某些能在甜橙上增殖的CTV株系在凤凰柚上的增殖受到了抑制。
For the preliminary study on influence of different citrus cultivars on the composition of Citrus tristeza virus（CTV）isolates, the Hinf Ⅰ RFLP and SSCP of p25 gene of 9 CTV isolates collected from sweet orange shown stem-pitting symptom were comparatively analysed as those isolates were graft inculated from Xinhui sweet orange to Fenghuang pummelo.The results of RFLP revealed that the Hinf Ⅰ RFLP group of the isolates CT90 and CT20 were kept the same as in Xinhui sweet orange and in Fenghuang pummelo; RFLP groups of other 6 isolates shown mixed groups in Xinhui sweet orange and single group in Fenghuang pummelo. DNA bands of p25/SSCP of the isolate CT90 kept the same as in Xinhui sweet orange and in Fenghuang pummlo but DNA bands of other 8 isolates maintained in Fenghuang pummlo were less than that of which maintained in Xinhui sweet orange.It seems that the replication of some composition of above 8 CTV isolates collected from sweet orange were inhibited in Fenghuang pummelo.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第5期1098-1102,共5页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30471205)
重庆市科委攻关专项(2005AB1010)
关键词
柑橘
柑橘衰退病毒
株系构成
寄主
Citrus
Citrus tristeza virus
Composition of isolate
Host
