摘要
目的:通过观察内毒素休克大鼠脑皮质中TLR4、IκBα和IL-18 mRNA的表达及地塞米松(Dex)对其的影响,探讨内毒素引起脑组织损伤的分子机制。方法:18只Wistar大鼠随机分为实验对照组(Control)、内毒素休克组(LPS)和地塞米松组(LPS+Dex),每组6只。除对照组外,大鼠静脉注射内毒素(4mg.kg-1),对照组大鼠静脉注射等量葡萄糖溶液;4h后检测脑皮质中TLR4、IκBα和IL-18mRNA的表达。结果:LPS+Dex组平均动脉压明显高于LPS组(P<0.01),大鼠存活率亦明显高于LPS组(P<0.01)。LPS+Dex组TLR4、IL-18 mRNA表达均明显低于LPS组(P<0.05),而IκBαmRNA表达明显高于LPS组(P<0.05)。结论:Dex能下调脑组织中TLR4 mRNA表达,而上调IκBα mRNA表达,对中枢神经系统具有一定的保护作用。
Objective To explore the molecular mechanism of brain tissue injury induced by endotoxin and observe the effects of Dex on TLR4, IκBα and IL-18 mRNA expressions of cortex in the endotoxic shock rats. Methods Eighteen Wistar rats were randomly divided into LPS, LPS＋DEX and control group （n=6）. Except control group, all the rats were administered with LPS （4 mg · kg^-1） by intravenous injection. The rats in control group were given glucose solution at same volume. The TLR4, IκBα and IL-18 mRNA expressions were assayed 4 h after intravenous injection. Results The mean arterial pressure （MAP） and the survival rate in LPS＋ DEX group were significantly higher than those in the LPS group （P〈0.01）. TLR4 and IL-18 mRNA expressions in LPS＋DEX groups were obviously lower than those in LPS group （P〈0.05）. The IκBα mRNA expression in LPS ＋DEX group was significantly higher than that in LPS group （P〈0.05）. Conclusion Dex may down-regulate TLR4 expression and up-regulate IκBα expression in the brain tissue, and has protective effect on central nervous system.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第5期841-844,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅自然科学基金资助课题(200505139)
