摘要
前期研究发现CaMBP－10参与植物细胞对生长素应答反应的调节，但调节机理有待阐明．本文表明CaMBP－10是由于影响质膜H+－ATPase活性，进而影响了质子外泌和细胞伸长．并进一步证明，它对质膜H+－ATPase活性的调节是通过介导该酶磷酸化而实现的，最终调节了细胞对激素的应答．
aM BP-10(BP-10) is a novel calmodulin (CaM) endogenous binding protein that wasobserved and isolated in plant by our research group. In preliminary studies, it was shownthat BP-10 was involved in the regulation on the responses of coleoptile to auxin. The present study shows that BP-10 inhibits cell responses to auxin through inhibiting the activityof plasma membrane H+-ATPase (PM H+-ATPase). In order to elucidate the regulatorymechanism of PM H+-ATPase activity by BP-10, the relationship between phosphorylation and activity of PM H+-ATPase has been investigated in vivo. The results prove thatBP-10 regulates the activity of PM H+-ATPase by mediating phosphorylation of the enzyme, as a result it affects cell responses to IAA.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
1997年第2期101-103,共3页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
国家自然科学基金
关键词
钙调素结合蛋白
生长素
应答反应
植物细胞
calmodulin
calmodulin binding protein
auxin
PM H^+-ATPase
phosphorylation
