摘要
用同步荧光技术研究了细胞色素Ｃ与促进剂胱氨酸的相互作用，分别监测了二者相互作用时细胞色素Ｃ中色氨酸残基和酪氨酸残基的荧光光谱的变化和二种氨基酸残基的荧光强度随时间的变化。实验结果表明，胱氨酸分子与细胞色素Ｃ的赖氨酸残基缓慢结合，二者相互作用的最终结果使细胞色素Ｃ分子的构象发生了微小的变化。
The interaction of cytochrome C with cystine as promoter was studied by synchronous fluorescence spectroscopy.After cystine was added into cytochrome C solution,the synchronous flurorescence spectra of tyrosine and tryptophan residues in cytochrome C were monitered with time.The results indicated that the lysine residues in cytochrome C molecules combine with cystine slowly.It induced the small conformational change of cytochrome C molecules.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
1997年第1期108-112,共5页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金
关键词
细胞色素C
胱氨酸
同步荧光光谱
Cytochrome C, Cystine, Synchronous fluorescence spectroscopy
