摘要
针对5’-单磷酸腺苷(5’-AMP)在溶析结晶中易出现爆发结晶的问题,分别采用静态法和激光法测定了293—323 K下5’-AMP在纯水以及溶剂体积比为0.3—2.0的乙醇-水体系中的溶解度和超溶解度,以指数经验关联式建立了超溶解度关联式,并在试验条件下考察了温度、乙醇流速、pH值和搅拌速率对介稳区宽度的影响。运用中心复合设计预测了5’-AMP在本溶析结晶体系中介稳区间,并用扫描电镜观察了5’-AMP在结晶条件优化前后晶体形态的变化情况,为工业生产中提高结晶产品质量提供了理论指导。
Qualitative data on the solubility and supersolubility for binary system 5＇-AMP ＋ H2O solution and ternary system 5＇-AMP ＋ H2O ＋ ethanol solution was determined at 293-323 K in the volume fraction ethanol range of 0.3-2.0, which were essential for solvent-out crystallization studies. The supersolubility of 5＇-AMP fulfills well the empirical formula in both pure water and H2O ＋ ethanol solution. Temperature, ethanol addition rate, pH levels and rotate speed of stirrer were being recognized as nonvasive control parameter for adenosine 5＇-monophosphate crystallization. The metastable zone was obtained under the conditions of temperatures, ethanol addition rate, pH levels and rotate speed of stirrer and estimated by using Central Composite Designs in the solvent-out crystallization process, and the variation of crystal habit of 5＇-AMP was studied by scanning electron microscope after optimization of the crystallization process. All the results were important for the control of industrial crystallization of 5＇-AMP.
出处
《化学工程》
EI
CAS
CSCD
北大核心
2007年第10期16-19,共4页
Chemical Engineering(China)
基金
国家'十五'科技攻关项目
江苏省'333新世纪科学技术带头人培养工程'(20027)
教育部<跨世纪优秀人才培养计划>项目
关键词
5'-AMP
结晶
溶解度
超溶解度
介稳区
5＇-AMP
crystallization
solubility
supersolubility
metastable zone
