摘要
目的研究Wnt信号中β-catenin基因第3外显子突变及蛋白异常表达在胰腺实性-假乳头状瘤（SPTs）发生中的作用。方法①对15例SPTs石蜡包埋组织进行DNA提取，PCR扩增，PCR产物纯化、测序，对β-catenin基因第3外显子进行突变分析。②采用免疫组化PV6000法检测SPTs组织中β-catenin蛋白的表达。结果①β-catenin基因第3外显子在15例SPTs组织中有12例发生了一个碱基的错义突变，突变率为80．0％，表现在密码子32位（5例）、33位（3例）和37位（4例）。②15例SPTs组织中有13例β-catenin蛋白呈细胞核表达，异常表达率为86．7％。结论Wnt信号中β-catenin基因突变及蛋白核内聚集在SPTs发生中起重要作用。
Objective To investigate the role of mutation of the 3^rd exon of β-catenin and abnormal expression of protein in occurrence of SPTs. Methods The pancreatic tissue extracted from 15 patients with SPTs were embedded with paraffin for DNA extraction, PCR amplification and purification and sequencing of PCR product. The mutation of the 3^rd exon of β-catenin was analyzed. The expression of β-catenin protein was determined with immunohistochemical PV6000 method. Results The vicarious mutation of one base in the 3^rd exon of β-catenin occurred in 12 of the 15 patients with SPTs （80. 0%）. The mutation located at the 32 in 5 cases, 33 in 3 and 37 in 4 of the codon. Conclusions The mutation of β-catenin gene and intranuclear accumulation of β-catenin protein play an important role in occurrence of SPTs.
出处
《中华肝胆外科杂志》
CAS
CSCD
2007年第10期705-708,共4页
Chinese Journal of Hepatobiliary Surgery
