摘要
目的研究白细胞过滤器清除血液回收机漂洗后血液中残存肿瘤细胞的效果,并观察过滤后红细胞的形态学及其Na+-K+-ATP酶活性的改变。方法选取胃肿瘤SGC-170细胞以及肠肿瘤LOVO细胞进行体外培养,待细胞消化后制成单一细胞悬液;采集非肿瘤患者术野血液,经过滤、离心、漂洗收集浓缩红细胞。将细胞悬液中的肿瘤细胞计数后混入浓缩的红细胞中,混匀,再用血液回收机过滤、离心、漂洗,将漂洗后得到的浓缩红细胞用白细胞过滤器过滤,观察过滤对血样中肿瘤细胞的影响并与未经过滤处理的对照组进行比较,同时测定红细胞中的Na+-K+-ATP酶,观察过滤对红细胞的影响。结果过滤后的血样中未发现肿瘤细胞,而对照组血样中可见到肿瘤细胞。培养14天后观察,经白细胞过滤器过滤后的血样未见肿瘤细胞生长,而对照组血样中有大量肿瘤细胞生长。过滤后的红细胞形态学未发生明显改变,Na+-K+-ATP酶含量与对照组相比无显著性差异(P>0.05)。结论应用自体血液回收机配合白细胞过滤器,可有效去除血液中的肿瘤细胞,且对红细胞形态及其中Na+-K+-ATP酶活性无明显影响。
Objective To explore and evaluate the efficiency of the leucocyte depletion filter on eliminating tumor cells from blood, and its influence on the morphology and function of red blood cells. Methods Blood from operation field was collected, filtered, centrifuged and washed. Intestinal cancer cells （LOVO） and gastric cancer cells （SGC） were cultured in vitro and mixed with the collected blood. Blood contaminated with known number of tumor cells was then centrifuged and washed with cell retriever, and then the mixture was filtered with leucocyte depletion filter. The amount of residual tumor cells in filtered blood was counted after trypan blue staining before and after culturing. Unwashed and unfiltered blood containing tumor cells were used as control and were also cultured. The morphology and Na^＋-K^＋ -ATPase activity of treated red blood cells was assessed. Results Tumor cells were detectable after only being washed with cell retriever and grew well after culturing for two weeks. No tumor cells were found after both washed with cell retriever and filtered with leucocyte depletion filter, and no tumor cell grew two weeks after culturing. No change was found in morphology and Na^＋ -K^＋ -ATPase activity of red blood cells after being washed and filtered. Conclusion Cell retriever is not able to eliminate tumor cells from blood completely. Further filtration with leucocyte depletion filter is capable of completely eliminating the residual tumor cells in blood. The morphology and Na^＋ -K^＋ -ATPase of red blood cells do not change after being washed with cell retriever and being filtered with leucocyte depletion filter.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2007年第10期1034-1035,共2页
Medical Journal of Chinese People's Liberation Army
