摘要
本文介绍了免疫性流产患者在免疫治疗后的反应。我们对75例流产3次或以上的妇女进行了配偶白细胞免疫治疗。在治疗前后分别采用液流细胞仪(FCM)和传统的微量淋巴毒试验(MCX)测定血清抗白细胞抗体,并以混合淋巴细胞培养(MLC)测定其血清抑制效应。结果表明免疫治疗后多数患者能产生抗白细胞抗体和MLC抑制效应,提示免疫治疗可促使机体产生封闭性抗体以增强免疫保护作用。我们对其中22例并测定了治疗前后对非配偶白细胞的抗体反应,发现多数患者FCM阳性,部分MCX阳性。提示这种抗体并非针对个体特异性MHC Ⅰ型或Ⅱ型抗原,抗体对配偶及非配偶白细胞的反应并不能单纯以具有相同的HLA抗原来解释。实验表明FCM法敏感性高,能准确、快速地检出抗体,明显优于MCX与MLC。
Seventy-five women with three or more recurrent spontaneous abortions were tested for anti-leukocyte antibodies by microcytotoxicity (MCX), flow cytometry (FCM) and mixed lymphocyte culture reactivity (MLC) before and after paternal leukocyte immuni- zation. 22 women of this group had parallel studies addressing the specificity of their responses by utilizing a panel of HLA typed 3rd-party leukocytes in all assays. We report: 1) Anti-leukocyte antibody determination by FCM is twice as sensitive as the MCX; 2) The anti-T cell antibody binding to spouse and 3rd-party can not be explained by class I antigen sharing alone. 3) The same applies to women who developed anti-B cell antibodies. 4) Some women who failed to produce anti-B cell antibodies to spouse show strong anti- body binding to 3rd-party leukocytes. 5) The presence of both anti-T and anti-B cell antibodies to paternal leukocytes correlates strongly with maternal serum mediated blocking of MLC whether paternal or 3rd-party stimulator cells are used. Studies are in progress utilizing FCM and monoclonal reagents to determine if the maternal antibodies produced following immunization is directed to TLX, private or public class I or II MHC antigens.
关键词
流产
习惯性
预防
控制
ABORTION
HABITUAL/immunology
ABORTION
HABITUAL/prevention and control
IMMUNOTHERAPY
