摘要
目的:从污水处理池的污泥中分离得到去除氨氮效果较好的菌株,对其进行分类鉴定和去除氨氮的最佳条件的研究。方法:根据维诺格拉斯基柱法分离出几种纯菌株,选取其中脱氮效果较好的一株,经形态观察和生理生化实验,鉴定其分类地位。研究不同培养条件对该菌生长的影响,用滴定法测定不同条件下其对氨氮的去除率。结果:获得一脱氮菌株P6,初步推测该菌为产碱杆菌。其最适生长条件为:温度29℃,初始pH值7.5以及摇床(100r/min)培养,且在温度29℃、废水的初始pH值11、接种量与废水体积的比例为1.2g/150mL、添加乙酸钠浓度为0.1%、废水的氨氮浓度为600mg/L和摇床(100r/min)的条件下,3d后该菌对氨氮的去除率达到45%。结论:P6菌有一定的去除氨氮能力,具有应用价值。
A strain which was good at removing nitrogen- ammonium was isolated from the sludges of fertilizer plant. Then the strain was identified and its optimum conditions were studied. Methods： Several strains were isolated with the method of Winogradsky column. The strain which had ability of nitrogen - ammonium removal was identified according to morphological observation, biochemical and physiological test. And it was cultivated on various conditions..When nitrogen- ammonium amount on various conditions was mensurated with the method of titration. Results：The strain named as P6 was identified as Alccdigenes sp.. The optimum conditions for the growth of the strain were： temperature 29℃, initial pH 7.5 and shaking at 100r/min. Under optimum conditions as temperature 29℃, initial pH of wastewater 1 l, the proportion of biomass and bulk of wastewater 1.2g/150mL, CH3COONa amount 0.1% ,nitrogen- ammonium amount 600mg/L and shaking at 100r/min the nitrogen - ammonium removal rate by strain P6 reached 45% after 3 days. Conclusion： This study provided a good strain for the further studies on nitrogen-ammonium removal.
出处
《生物技术》
CAS
CSCD
2007年第5期64-68,共5页
Biotechnology
基金
安徽省自然科学基金项目(No.070413132)
安徽大学人才队伍建设项目资助
关键词
产碱杆菌
氨氮
废水
去除率
Alcaligenes sp.
nitrogen - ammonium
wastewater
removal rate
