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拟南芥转录因子CBF1基因杂交狼尾草的转化 被引量：11

Transformation of Transcription Activator CBF1 Gene of Arabidopsis into Hybrid Pennisetum
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摘要 CBF1转录激活因子是一类存在于拟南芥中受低温诱导的反式作用因子,能有效提高植物抗低温、抗干旱的能力。因此以拟南芥叶片为材料,通过PCR方法成功地克隆CBF1转录因子基因,并将其连接到植物表达载体pBI121上,通过农杆菌介导法转化杂交狼尾草叶片,成功获得转基因再生植株。 Transcription activator CBF1 is a trans-activating factor in arabidopsis, which can be induced by low temperature. The CBF1 gene is cloned, which is derived from Arabidopsis with PCR. Then CBF1 cDNA and plant expression vector pBI121 are conjugated to construct the recombinant plasmid pBI121-CBF1. With agrobacteriummediated transgene technique, the authors successfully introduce the arabidopsis transcriptional factor gene CBF1 into hybrid pennisetum, and reproduce the transgenic hybrid pennisetum.
作者 王凭青 李志中 晁跃辉 鲁浪 张宝云
机构地区 重庆大学生物工程学院
出处 《重庆大学学报（自然科学版）》 EI CAS CSCD 北大核心 2007年第10期134-137,共4页 Journal of Chongqing University
基金 重庆市科技攻关资助项目(CSTC2005AC1021)
关键词 CBF1基因 抗性 转化 杂交狼尾草 CBF1 gene tolerance transform hybrid pennisetum
分类号 Q943.2 [生物学—植物学]
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参考文献9

  • 1GILMOUR S J, ZARKA D G, STOCKINGER E J, et al.Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in coldinduced COR gene expression[J]. Plant J,1998,16: 433.
  • 2JAGLO-OTrOSEN K R, GILMOUR S J, ZARKA D G, et al. Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance [ J ]. Science, 1998,280 (5360) :104-106.
  • 3MEDINA J, BARGUES M, TEROL J, et al. The arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression Is regulated by low temperature but not by abscisic acid or dehydration[J]. Plant Physiol, 1999, 119: 463.
  • 4SHINWARI Z K, NAKASHIMA K, MIURA S, et al. An arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expression [ J ]. Biochern Biophyses Rommun, 1998, 250( 1 ) : 161 -170.
  • 5STOKINGER E J, GILMOUR S J, THOMASHOW M F. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the Crepeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit[ J ]. Proc Nail Acad Sci USA, 1997, 94 (3) : 1035 -1040.
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  • 7LEE S C, HUH K W, AN K, et al. Ectopic expression of a cold-inducible transcription factor, CBF1/DREBlb, in transgenic rice[J]. Mol Cells, 2004, 18(1) :107-114.
  • 8BADAWI M, DANYLUK J, BOUCHO B, et al. The CBF gene family in hexaploid wheat and its relationship to the phylogenetic complexity of cereal CBFs [ J ]. Mol Genet Genomics, 2007, 277(5):533-554.
  • 9BENEDICT C, SKINNER J S, MENG R, et al. The CBF1- dependent low temperature signalling pathway, regulon and increase in freeze tolerance are conserved in Populus spp[J]. Plant Cell Environ, 2006, 29(7) :1259-1272.

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重庆大学学报（自然科学版）
2007年 第10期

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