摘要
目的时间分辨荧光免疫分析技术(TRFIA)是当前标记免疫分析研究应用领域的热点之一,具有高灵敏性和高特异性,用于生物分子的超微量检测。本研究者在建立一种灵敏度高、特异性强、操作简便的检测方法。方法以Eu-DTTA{N1-(P-异硫氰基苄基)-二乙三胺四乙酸铕钠}为示踪物,建立了用TRFIA检测乙型肝炎血清学标志物乙型肝炎病毒表面抗原的方法。结果所建立的标准曲线分析范围分别为0.2～300ng/ml,分析灵敏度为0.05ng/ml,检测参考标准品的批内变异系数均小于10%,与免疫放射测定同时定量检测多份血清样本,相关系数高达95%。结论TRFIA分析范围宽,灵敏度高,操作简便,具有优越的定量检测能力,价格适中,十分适合临床推广应用。
A convenient,highly sensitive and highly specific method using time-resolved fluoro immunoassay（TRFIA）for quantitative detection of hepatitis B virus is described.Using EU-DTTA as the tracer molecule,the assay showed a detection range of the standard curves of 0.2-300 ng/ml for HbsAg with a sensitivity of 0.05 ng/ml.The within-run coefficient of variations for standard samples were less than 10%,and the correlation coefficient between radioimmunoassay and TRFIA assay results reached 95% for the same sample,demonstrating the advantages of TRFIA for its wide detection range,high sensitivity and simple operation and its great potentials for clinical use.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第10期1575-1576,共2页
Journal of Southern Medical University
关键词
时间分辨荧光免疫分析
病毒性肝炎
表面抗原
time-resolved fluoroimmunoassay
hepatitis B virus
hepatitis B virus surface antigen
