摘要
探讨乳腺癌多药抗药（ＭＤＲ）相关药物的体外敏感性与ｍｄｒ－１基因表达的关系，为乳腺癌的合理化疗提供理论依据。方法采用体外ＭＴＴ药敏试验及多聚酶链反应（ＲＴ－ＰＣＲ）技术，检测了５６例乳腺癌病人的原代乳腺癌细胞对ＭＤＲ相关药物的敏感性，及乳腺癌组织的ｍｄｒ－１基因表达水平。结果体外抗药乳腺癌的ｍｄｒ－１基因阳性表达率均明显升高；Ｉｍａｘ％与ｍｄｒ－１基因表达水平无关；而ＩＣ５０与ｍｄｒ－１基因性的相关性具有统计学意义。
Objective To study the relationship between in vitro chemosensitivity of human breast cancer to MDR related drugs and experssion of mdr 1 gene.Methods We measured the chemosensitivity of 56 human breast cancers to MDR related drugs by MTT assay in vitro and level of mdr 1mRNA in these samples by RT PCR technique.Results The expression of mdr 1gene in the breast cancer resistant to drugs in vitro was increased significantly.The level of mdr 1mRNA was not correlated with I max %,but correlated with IC50 statistically.Conclusion These results support that the increased expression of mdr 1 gene is a major cause of resistance to MDR related drugs.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第5期371-373,共3页
National Medical Journal of China
关键词
乳腺肿瘤
MDR-1
基因表达
聚合酶链反应
抗药性
Breast neoplasma Drug tolerance MTT Colorimetry gene Expression Polymerase chain reaction
