摘要
参照GenBank登录的猪白细胞介素-15(IL-15)基因序列自行设计1对特异性引物,运用RT-PCR技术从由刀豆蛋白A(ConA)诱导培养的荣昌猪外周血淋巴细胞扩增出猪IL-15基因的完整CDS序列,全长489 bp。同源比对结果表明,荣昌猪IL-15基因与已公布的2个猪种IL-15基因核苷酸同源性均为99.4%,与哺乳动物的同源性较高,与鸡的同源性较低。密码子偏爱性分析显示,荣昌猪IL-15基因在密码子使用上存在一定的偏爱性;分子进化分析结果表明其与人及哺乳动物IL-15基因的进化关系较近,而与禽类鸡的IL-15基因进化距离较远;结构域预测分析显示其与人的IL-15存在很大的相似性,可能在功能上有相似性。
Using stimulated with ConA peripheral blood lymphocyte and a set of specific primers,the Interleukin-15 gene of swine was amplified by PCR. The primers were designed based on the published sequences of the swine IL-15 gene. The 489bp amplified DNA fragment was cloned into pMD18-T vetor. The results showed that the sequence identities were 99 between amplified IL-15 gene and that from GenBank. Bioinformatics analysis indicated some Codon preference ,Phylogenetic tree analysis indicated that the have evolutionary relationship with mammalian IL 15,however showed distinct difference with chicken IL-15. There are distinct resemblance between pig IL 15 and human IL-15 by structural domain prediction analysis .
出处
《中国畜牧兽医》
CAS
2007年第10期37-40,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家"973"项目(2004CCA011800)
教育部长江学者和创新团队发展计划资助(IRT0555)
