摘要
彩纹海棠叶片培养在MS基本培养基中,研究植物的激素、培养基的物理性质对器官形成的影响及试管苗移栽技术等.试验结果表明细胞分裂素BA促进芽的形成和增殖。BA和NAA配合使用,对叶片形成芽有增效作用。通过继代培养,能繁殖大量无根苗。将无根苗转入含有NAA0.2mg/L或IBA ling/L的1/2MS培养基中,诱导生根形成完整植株。诱导叶片形成芽应采用固体培养基;而液体静置培养则有利于促进芽发育成苗和生根。试管苗移栽获得成功,幼苗生长良好。
The leaf explants of Begonia masoniana var. maculata were grown on MS basal medium, the effects of plant hormones and physical properties of medium on organognesis and trasplanting of test-tube plantlets were studied. The results showed that BA stimulated the bud formation and proliferation. In using the combination of BA and NAA, the efficiency of bud formation of the leaf explants was increased. Througth subculture numerous plantlets could be propagated, when the shoots were transferred into 1/2 MS basal medium with 0.2 mg/ 1 NAA or 1mg/l IBA, they developed into whole plantlets with root systems. Agar media stimulated buds formation of leaf explants, but liquid media beneficial bud development and root formation. Transplanting of test-tube plantlets in the soil was succeeded and plants were grown well.
出处
《广西植物》
CAS
CSCD
北大核心
1990年第2期161-167,共7页
Guihaia
关键词
彩纹海棠
繁殖
Begonia masoniana var. maculata
rapid propagation
plant hormones
organogens's
