摘要
对一对籼(Oryza sativa ssp.indica)粳(O.sativa ssp.japonica)(“圭630”/“02428”)杂种进行花药培养获得81个双单倍体(DH),构建了有233个RFLP标记的水稻遗传连锁图谱。对各DH系的“圭630”等位基因组频率及图示基因型进行了分析。结果表明,该DH群体的平均基因组频率为49％,各DH系分布在29.3％～78.6％之间,集中分布在44％～49％之间;有130个RFLP标记出现显著的偏分离,偏父本与偏母本分离的标记数基本相等;同向偏分离的标记常集中在一些染色体或染色体的某些区段;各染色体的平均基因组频率分布在29％～65％之间,出现明显的偏父或偏母分离;一些DH中出现其特征完全来于某一亲本的染色体,可能与这些染色体在减数分裂期的同源配对和交换有关。
A rice RFLP map with 233 loci based on a population of 81 doubled haploids (DH) from the indica/japonica hybrid of 'Gui 630'/'02428' was used to analyse the genome ratios and graphical genotypes of DH lines. The 'Gui 630' genome ratio of the individual DH lines varied from 29.3% to 78.6% with an average of 49% while the ratios of most DH lines ranged between 44% and 49% . Of the mapped RFLP markers 130 showed skewed segregations with a significant deviation from the expected monogenic ratio, but the numbers of the markers deviated towards male and female parent were approximately equal. It was found that the markers with the segregation deviation in the same direction tend to cluster on some chromosomes and some of their regions. The average 'Gui 630' genome ratios of different chromosomes in the DH population varied greatly between 29% and 65 % . In addition, several chromosomes were inherited completely from either one of the parents in some DH lines, indicating the rare occurrence of crossover along the pairing homologous chromosomes during meiosis.
关键词
水稻
籼粳杂种
双单倍体
基因型
RFLP
Rice, Hybrid of indica and japonica, Doubled haploid, Graphical genotype, Genome ratio, Distorted segregation
