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p38丝裂原活化蛋白激酶在肠缺血再灌注损伤大鼠炎性反应中的作用 被引量：4

Role of p38 mitogen-activated protein kinase in inflammatory response to intestinal ischemia-reperfusion injury in rats
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摘要 目的探讨p38丝裂原活化蛋白激酶（p38MAPK）在肠缺血再灌注损伤大鼠炎性反应中的作用。方法健康成年雄性Wistar大鼠30只,随机分为3组（n=10）：假手术组（S组）、缺血再灌注组（I/R组）和p38MAPK抑制剂SB203580组（SB组）。采用夹闭肠系膜上动脉（SMA）的方法制备肠缺血再灌注损伤模型。I/R组和SB组夹闭SMA 1 h再灌注6 h,SB组缺血前30 min经股静脉注射p38MAPK特异性抑制剂SB203580 100μg/kg。于再灌注6 h时,处死大鼠,测定血浆肿瘤坏死因子-α（TNF-α）浓度、双胺氧化酶（DAO）活性及小肠组织TNF-α含量、p38MAPK、细胞间粘附分子-1（ICAM-1）表达水平,在光镜下观察小肠组织病理学,并进行小肠组织损伤程度评分。结果与S组比较,I/R组血浆TNF-α浓度、DAO活性及小肠组织p38MAPK、ICAM-1表达、TNF-α含量、小肠组织损伤程度评分升高,SB组血浆DAO活性、小肠组织ICAM-1表达、TNF-α含量及小肠组织损伤程度评分升高（P〈0.05）;与I/R组比较,SB组血浆TNF-α浓度、DAO活性及小肠组织p38MAPK、ICAM-1表达和TNF-α含量、小肠组织损伤程度评分降低（P〈0.05）。结论p38MAPK参与了肠缺血再灌注损伤大鼠炎性反应。 Objective To investigate the role of p38 mitogenoactivated protein kinase （p38MAPK） in inflammatory response to intestinal ischemia-reperfusion （I/R） injury. Methods Thirty adult male Wistar rats weighing 200-250 g were randomly divided into 3 groups （ n = 10 each） ： Ⅰ control group （C） ; Ⅱ I/R group and Ⅲ SB203580 ＋ I/R （SB）. The animals were anesthetized with intraperitoneal 7% chloral hydrate 350 mg/kg. Intestinal I/R was produced by occluding superior mesenteric artery for 1 h followed by 6 h reperfusion in group Ⅱand Ⅲ . In group Ⅲ SB203580 （ a selective p38MAPK inhibitor） 100μg/kg was given intravenously 30 min before ischemia. Blood samples were collected at the end of 6 h reperfusion for determination of plasma TNF-α concentration and diaminoxidase （DAO） activity. A small segment of ileum was obtained for determination of TNF-α content and expression of p38MAPK and ICAM-1 protein and microscopic examination. Results The extent of damage to small intestine was significantly greater in group I/R and SB than in group C. The plasma DAO activity and TNF-α concentration and the expression of p38MAPK and ICAM-1 protein and TNF-α content in small intestine tissue were significantly increased in group I/R and SB as compared to control group. SB203580 pretreatment significantly attenuated the I/R-induced deleterious changes. Conclusion p38MAPK is involved in the inflammatory response to intestinal I/R injury in rats.
作者 刘永芳 刘先义 刘志刚 丰新民 王锋
机构地区 武汉市第一医院麻醉科 武汉大学人民医院麻醉科
出处 《中华麻醉学杂志》 CAS CSCD 北大核心 2007年第9期839-841,共3页 Chinese Journal of Anesthesiology
关键词 P38丝裂原活化蛋白激酶类 再灌注损伤 肠 炎症 p38 Mitogen-activated protein kinases Reperfusion injury Intestines Inflammation
分类号 R686 [医药卫生—骨科学]
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参考文献10

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二级参考文献33

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共引文献25

同被引文献54

引证文献4

二级引证文献26

中华麻醉学杂志
2007年 第9期

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