摘要
目的研究不同疗程高压氧干预对大剂量激素用药后大鼠股骨近端组织学、BMP-2及其受体表达水平的影响。方法对90只4月龄雄性Wister大鼠进行大剂量地塞米松肌肉注射6周后,将其随机分为3组,即对照组(A组)、1个疗程组(B组)和3个疗程组(C组)。以B、C组高压氧治疗开始后4,8,12周作为观察时间点,对3组大鼠股骨近端骨质进行组织学观察,同时检测BMP-2和BMP-RI表达水平的变化。结果B组、C组股骨髓腔内血管数量多于A组,骨小梁较A组宽,骨皮质较A组厚,3组骨小梁/髓腔面积比(TCR)、皮质/髓腔面积比(CCR)、BMP-2平均染色面积百分比(DAP)、BMP-2染色平均光密度值(OD)、BMP-RI含量(BRC)在各观察时间点的关系均为:C组>B组>A组,各组间差异均具有统计学意义(P<0.01);A组TCR、CCR、DAP、OD、BRC均随时间延长呈降低趋势,但在3个观察时间点间的差异无统计学意义(P>0.05),而B组、C组上述5个指标则呈升高趋势,其中BRC在各时间点间的差异均有统计学意义(P<0.01),其它4项指标在4周与8周时、4周与12周时的差异均有统计学意义(P<0.01),而在8周与12周时的差异无统计学意义(P>0.05)。3组大鼠空骨陷窝率(ER)间差异具有统计学意义(P<0.01);A组的ER有随时间延长而增高的趋势,但在3个观察时间点间的差异无统计学意义(P>0.05);而B组、C组ER随时间延长而降低,在4周与8周时、4周与12周时的差异均具有统计学意义(P<0.01),而8周与12周时的差异不具有统计学意义(P>0.05)。结论停止使用大剂量激素后,大鼠股骨近端组织的病理改变继续存在,并且在早期有加重的趋势;高压氧干预可以抑制甚至逆转这些病理改变;3个疗程高压氧治疗效果优于1个疗程干预。
Objective To evaluate the effects of different sessions of hyperbaric oxygen （ HBO ） therapy on proximal femur after a short period of high dosage of glucocorticoid application in rat model by assessing histology, expression of BMP-2 and its receptor. Methods Ninety 4-month-old Wistar male rats were administered with high dosage of dexamethasone by intramuscular injection for 6 weeks, and then were divided into 3 groups： a control group （Group A） and two experimental groups （Groups B and C）. Groups B and C were treated with HBO once daily for 10 days and 30 days, respectively. After 4, 8 and 12 weeks of HBO therapy, histological observation, and immuno- histochemistry examination of expression of BMP-2 and its receptor （ BMP-RI ） of the upper 1/3 of femur were conducted, respectively. Results There were significantly more vessels of cavity in Groups C and D than those of Group A, the bone trabeculae and cortical bone of Groups C and D were thicker than those of Group A. The order of the three groups with regard to area ratio of bone trabeculae and medullary cavity （TCR）, area ratio of cortical bone and medullary cavity （CCR）, average percentage of dyed areas （DAP） and average optical density （OD） of BMP-2 and contents of BMP-RI （BRC） of proximal femurs are Group C 〉 Group B 〉 Group A, the differences among the three groups were statistically significant （ P 〈 0.01 ）. There was a trend of decreasing in Group A with regard to TCR, CCR, DAP, OD, BRC, but the differences among the 3 phases were not significantly different （P 〉 0.05 ）. TCR, CCR, DAP, OD, BRC in Groups B and C showed trends of increasing, the differences in terms of the contents of BMP-RI among the 3 phases were statistically significant （ P 〈 0.01 ）. As of the empty rate of bone lacunae （ ER）, there is significant difference among the three groups （P 〈 0.01 ）. The ER in group A showed a trend of in-creasing with time, however, there was no significant difference among the 3 phases （P 〉 0.05 ） ; the ER in Group B and C showed a trend of decreasing, thee difference between 4- and S-week and 4- and 12-week were significantly different （ P 〈 0.01 both） , except for that between the 8- and 12-week （ P 〉 0.05 ）. Conclusion After application of glucocorticoid for a short term, pathological changes maintained and showed trends of increasing in early stage. HBO can reverse these changes. The outcome of 3-course HBO therapy is better than that of l-course therapy.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2007年第9期586-589,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
高压氧
激素
股骨
骨形态发生蛋白
骨形态发生蛋白受体
Hyperbaric Oxygen
Glucocorticoid
Femur
Bone morphogenetic protein
Bone morphogenetic protein receptor
