摘要
国家禽流感参考实验室在2006年,从来自青、藏两省(区)3种野鸟及辽宁省的2种死亡的野鸟体内共分离到14株H5N1亚型高致病力禽流感病毒(High pathogenic avian influenza virus,HPAIV),比较发现其HA、NA基因核苷酸的同源率在97.9%～99.9%之间。所有病毒的HA基因的裂解位点均具有HPAIV特有的连续碱性氨基酸-RRRKKR-,并具有近年来H5N1亚型流行株所特有的NA基因颈部49～68位20个氨基酸缺失及NS基因80～84位5个氨基酸的缺失;遗传进化分析表明2006年野鸟病毒由2005年野鸟病毒进化而来,并形成独立的进化分支;辽宁省近2年的4株野鸟病毒亲缘关系较近,说明引起2005年该省锦州地区H5N1 HPAI疫情的病毒在该地区的野鸟体内继续存在。
Twelve highly pathogenic avian influenza viruses （HPAIV） of H5N1 subtype were isolated from 3 species of migratory birds in Qinghai and Tibet and 2 viruses from dead magpie and wild duck in Liaoning province in 2006. Sequence analysis showed that the HA and NA genes of these viruses shared 97.9 % to 99.9 % sequence identity with other viruses. All 14 viruses harbored a series of basic amino acids （-RRRKKR-） at the hemagglutin （HA） cleavage site similar to the majority isolates circulating during recent years, and had a 20-amino acid deletion in the neuraminidase （NA） stalk （residues 49-68）, Phylogenic analysis suggested that viruses isolated from wild bird in 2006 were originated from those in 2005, and likely have evolved to a separate clade of which 4 viruses from Liaoning formed a closer clade. These results indicated that the highly pathogenic avian influenza viruses remain exist in nature.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2007年第10期760-763,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家"十五"科技攻关禽流感病原学平台研究(2004BA519A20)
