摘要
目的:观察长期有氧运动后大鼠脂肪组织中TNF-α和PPARγ的变化,探讨运动对脂肪组织分化机制的影响。方法:SD大鼠随机分为对照组和运动组,运动组大鼠进行12周的有氧游泳运动。用ELISA法检测大鼠内脏脂肪组织中TNF-α含量。流式细胞仪检测脂肪组织中PPARγ蛋白表达量。结果:①实验后,运动组大鼠体重与对照组无差异,但脂体比显著低于对照组。②运动组大鼠与对照组大鼠相比,脂肪组织中TNF-α含量显著下降(运动组和对照组分别为8.22±2.62pg/mg和25.50±18.70pg/mg,P<0.01)。③运动组大鼠脂肪组织中PPARγ蛋白表达量显著升高(运动组和对照组分别为1.13±0.13和0.93±0.06,P<0.05)。结论:长期有氧运动后,脂肪组织中TNF-α含量下降而PPARγ含量升高,这说明长期有氧运动后脂肪组织可能处于脂肪合成和脂肪细胞分化能力升高的生理状态。
Objective ：To analyze the effect of long term aerobic exercises on the changes of TNF-α and PPARγ in adipose tissue and to explore the mechanism of adipose tissue differentiation. Method： Fourteen SD rats were divided into two groups, control group and exercises group. Exercise group rats performed 12 weeks aerobic swimming exercises. TNF-α concentration in visceral adipose tissue was tested by ELISA. PPARγ content in visceral adipose tissue was tested by flow cytometry. Result： TNF-α concentration in visceral adipose tissue of exercises group was lower than control group （8.22±2.62 and 25.50±18.70,P〈0.01）, but PPARγ content was higher than control group （1.13±0.13 and 0.93±0.06,P〈0.05）. Conclusion： TNF-α decreasing and PPARγ increasing in adipose tissue suggest after long term aerobic exercises adipose tissue may be in the physiological condition of increasing potential of fat synthesis and adipocytes differentiation.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2007年第9期779-781,共3页
Chinese Journal of Rehabilitation Medicine
基金
河北省自然科学基金项目(C2005000156)
关键词
有氧运动
脂肪组织
肿瘤坏死因子
过氧化物酶体增殖物激活受体
aerobic exercises
adipose tissue
tumor necrosis factor-alpha
peroxisome proliferator-activated receptors
