摘要
目的：建立简单、快速、灵敏的能同时分离测定防己及其制剂中防己诺林碱和防己碱的非水毛细管电泳方法。方法：采用非水毛细管电泳法，缓冲体系50mmol·L^-1醋酸铵-1．0％醋酸-20％乙腈的甲醇溶液，熔融石英毛细管（75μm×50．0cm），分离电压20kV，检测波长214nm，阳极进样。结果：防己诺林碱和防己碱在1．00～500mg·L^-1与峰面积线性关系良好。迁移时间的日内相对标准偏差分别为0．09％和0．59％，日间相对标准偏差分别为0．63％和1．9％；峰面积的日内相对标准偏差分别为0．45％和4．9％，日间相对标准偏差分别为2．3％和5．6％。防己中防己诺林碱和防己碱平均回收率分别为102％和105％，风痛安胶囊中防己诺林碱和防己碱平均回收率分别为94．6％和98．7％。结论：该方法简单、快速、准确、重复性好，可用于防己及其制剂的质量控制。
Objective： To establish a new method for the determination of fangchinoline and tetrandrine in Stephania tetrandra and Fengtongan capsule by noanqueous capillary electrophoresis. Method： Separation was carried out in an uncoated fused capillary （50 cm ×75 μm i. d. ） with a running buffer containing 50 mmol · L^-1 ammonium acetate, 1.0% acetic acid and 20% acetonitrile in methanol. A separation voltage of 20 kV and a UV detector wavelength at 214 nm were adopted. Sample was introduced from the anode. Result： The calibration ranges were 1.00, 500 mg · L^-1 for both analytes. Under the optimum conditions, the relative standard deviation （ RSD, n = 6） for the migration time of each analyte were 0. 09%, 1.9% （intra-day） and 0. 63%, 1.9% （inter-day） ; The RSD for the peak area of each analyte were 0. 45%, 5. 9% （intra-day） and 2. 3% , 5.6% （ inter-day）, respectively. The contents of the analytes were determined easily with average recoveries 102% for fangchinoline and 105% for tetrandrine in S. tetrandra and 94. 6% for fangchinoline and 98.7% for tetrandrine in Fengtongan capsules, respectively. Conclusion： The proposed method is simple, rapid, accurate and higher repeatable, and can be used to control of the quality of S. tetrandra and Fengtongan capsules.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第19期1992-1995,共4页
China Journal of Chinese Materia Medica
关键词
防己诺林碱
防己碱
非水毛细管电泳
防己
凤痛安胶囊
Fangehinoline
tetrandrine
nonaqueous capillary electrophoresis
Stephania tetrandra
Fengtongan capsules
