摘要
目的分析江苏省2003-2005年之间从环境样本中分离到的霍乱弧菌的核酸特征,及其差异的程度。方法将11株目的菌株的表型特征(包括血清型,耐药谱,生化特征以及El Tor霍乱弧菌的噬菌体分型)和核酸特征(包括霍乱毒素基因ctx-A、zot、ace、tcp-A的分布情况和脉冲场凝胶电泳指纹图谱)结合起来进行比较和分析。结果11株菌株中有10株O139群霍乱弧菌,1株ElTor小川;其中6株O139同时对5种以上抗生素耐受,2株O139对4种抗生素耐受,1株O139对2种抗生素耐受,另外1株O139(属于PFGE-C型)和1株小川分别只对一种抗生素耐受;11株霍乱弧菌的生化特征没有明显差异;8株O139同时携带4种毒力相关基因,1株0139只携带zot和ace,另外1株O139(05052)和1株小川未携带这4种基因;9株O139的脉冲场凝胶电泳指纹图谱聚为一类,另外1株O139和小川各自聚为一类。结论10株O139中有1株(属于PFGE-C型)的耐药谱、毒力相关基因分布及PFGE指纹图谱与其他9株不同,而与分离地点相同的小川相似,提示自然环境中的O139群霍乱弧菌存在着差异。
Objective The present study was conducted to analyze the NCA characteristics of Vibrio cholerae O139 swains isolated from environment-derived samples in Jiangsu from 2003 to 2005 and determine whether there was variance and its variation degree. Methods The combined comparison and analysis were conducted on the phenotype characteristics （including： serotype; drug fast spectrum; biochemical characteristics and the phage typing of E1 Tot Vibrio cholerae） and NCA characteristics （including： distribution state of choleratoxin genes ctx-A,zot,ace,tcp-A and pulsed field gel electrophoresis fingerprint） of 11 objective strains. Results Among 11 strains,10 strains were O139 isolates Vibrio cholerae and 1 strain was E1 Tot; of which 6 O139 strains resisted more than 5 antibiotics, 2 O139 strains resisted 4 antibiotics, 1 O139 strain resisted 2 antibiotics, and the last one O139 and one E1 Tot resisted only one antibiotic respectively; no significant variance in biochemical characteristics was found among all the strains, of which 8 strains O139 possessed 4 toxicity-related genes, 1 O139 strain only possessed zot and ace genes and another O139 （05052） strain and 1 E1 Tor had none of the 4 genes; There were two types of pulsed field gel electrophoresis fingerprints, 9 strains were classified to one type and the other two were to another type. Conclusion One O139 strain （Type PFGE-C） was different from the other 9 strains in drug-fast spectrum and toxicity-related gene distribution but was similar to one E1 Tor strain isolated at the same place, which indicated that variance could be found among the Vibrio cholerae O139 strains living in natural environment.
出处
《疾病监测》
CAS
2007年第8期510-513,共4页
Disease Surveillance
基金
江苏省疾病预防控制中心青年基金(基金号:JSQ200506)的资助
关键词
O139霍乱弧菌
核酸特征
表型特征
脉冲场凝胶电泳
Vibrio cholerae O139 strains
NCA characteristics
pulsed field gel electrophoresis fingerprint
