摘要
目的:通过观察活血化瘀中药对激素性股骨头缺血坏死血管内皮生长因子(VEGF)的表达,探讨活血化瘀法防治激素性股骨头缺血坏死的作用机制。方法:30只成年新西兰大白兔采用抽签法随机分为2组,正常对照组5只和激素造模组25只。激素造模组每周2次臀肌注射7.5mg/kg醋酸氢化泼尼松,6周后从激素造模组中抽签随机抽取5只与正常对照组5只同时处死,观察组织病理学变化和VEGF表达。再将其余激素造模组动物(20只)采用抽签法随机分为治疗1、2组与对照1、2组,每组5只。治疗1、2组用桃红四物汤(当归、川芎、赤芍、桃仁、红花、生地)7ml/kg灌胃,每天1次,对照1、2组等量生理盐水灌胃。于10周后处死治疗1组和对照1组动物,13周后处死治疗2组和对照2组动物,检测VEGF表达。结果:造模后VEGF的表达明显增加,与正常对照组比较差异有显著性意义(P<0.01),但随时间推移,其表达越来越弱。与治疗组比较对照组的VEGF表达明显减弱,有统计学差异(P<0.001)。结论:活血化瘀中药间接地促进了VEGF的表达,可以有效地改善股骨头微循环障碍。
Objective：To investigate the effect of traditional Chinese blood-activating and stasis-eliminating herbs on the expression of vascular endothelial growth factor（VEGF） in rabbits with glucocorticoid-induced femoral head necrosis, as well as to explore the mechanism of prevention and treatment of osteonecrosis of traditional Chinese blood-activating and stasis-elimina- ting herbs. Methods：In the study,30 New Zealand white rabbits were randomly divided into 6 groups：control group（5 rabbits） and glucocorticoid-induced model group （25 rabbits）. The rabbits in model group were administered with hydroxyprednisone acetate by intramuscular injection（twice a week）for 6 weeks, then 5 rabbits in the model group were selected randomly and killed and as well as the 5 rabbits in the control group, observe the histopathological changes and the expression of VEGF. Oth- er 20 rabbits in the model group were divided into four groups randomly ： treatment group 1 （ 5 rabbits）, treatment group 2 （5 rabbits）, control group 1 （5 rabbits）and control group 2 （5 rabbits）. The rabbits in treatment group 1 were administered with Chinese herbs Taohong Siwu decoction（桃红四物汤） for g weeks at a dose of 7 ml/kg,once a day,and then killed. The rabbits in treatment group 2 were administered with Chinese herbs Taohong Siwu decoction（桃红四物汤） for 7 weeks at a dose of 7 ml/kg,once a day ,and then killed. The rabbits in control group 1 were administered with normal saline for g weeks at a dose of 7 ml/kg,once a day,and then killed. The rabbits in control group 2 were administered with normal saline for 7 weeks at a dose of 7 ml/kg,once a day ,and then killed. Then the expression of VEGF was defected. Results：The expression of VEGF increased significantly after 6 weeks compared with the common group （ P 〈0. 01 ） , but its expression became weaker and weaker and eventually decreased significantly compared with treatment gruops in 10 weeks and 13 weeks （P 〈 0. 001 ）. Conclusion： The traditional Chinese blood-activating and stasis-diminating can indirectly promote the expression of VEGF and improve the microcirculation of avascular necrosis of femoral head.
出处
《中国骨伤》
CAS
2007年第9期601-602,共2页
China Journal of Orthopaedics and Traumatology
基金
福建省自然科学基金课题(编号:C0510024)
关键词
股骨头坏死
激素类
活血祛瘀药
血管内皮生长因子类
Femur head necrosis
Hormones
Blood act stasis remove drugs
Vascular endothelial growth factors
