摘要
目的检测小鼠胎鼠成骨细胞体外培养并将不同浓度雄激素干预后,OPG和OPGLmRNA表达的变化,探讨雄激素在成骨细胞介导破骨细胞分化、活化过程中的调控机制。方法分离得到小鼠胎鼠颅盖骨成骨细胞,培养传代并选择第二代细胞用于实验;对第二代成骨细胞实施含10-10mol/L、10-9mol/L、10-8mol/L3种浓度雄激素的培养液干预;抽提细胞RNA,采用RT-PCR方法半定量观察成骨细胞中OPG和OPGL基因mRNA表达的变化。结果实验选用的各浓度组均未出现细胞毒性反应,雄激素干预使成骨细胞中OPG基因表达上调,而OPGL与OPG比率随时间呈递减趋势。结论雄激素可以特异性地在转录水平调节成骨细胞中OPG和OPGL基因的表达。
Objective To investigate the effects of androgen on the expression of OPG and OPGL, and to further clarify the mechanism of regulation of androgens on osteoblast in the process of inducing osteoclast differentiation and activation. Methods The second-passage osteoblasts that were tested to be characterized as being of osteoblastic phenotype were used in experiments; androgen were derided into 3 groups of 10^-10 mol/L. 10^-9 mol/L, 10^-8mol/L and then exert influence on osteoblasts; mRNA expressions of OPG and OPGL were semi-quantified by RT-PCR. Results No cell toxicity was found in all concentrations we used in experiments. The expression of OPG in osteoblasts was significiantly increased and up-regulated by androgen, and androgen promoted a decrease in the ratio of OPGL and OPG with time. Conclusion Androgen can regulate the expression of OPG and OPGL mRNA in gene-level in osteoblasts.
出处
《中国骨质疏松杂志》
CAS
CSCD
2007年第2期77-81,共5页
Chinese Journal of Osteoporosis
基金
上海市高校科学技术发展基金项目(03BK26)
