摘要
[目的]研究花生胚小叶体细胞植株再生体系,为利用胚小叶进行外源基因遗传转化提供试验依据。[方法]以花生品种四粒红和改良海花的胚小叶为外植体,2种外植体取材方式(预培养和直接取材)为培养背景,探讨了不同取材方式下各个培养要素(基因型、培养基等)与脱分化、再分化的关系,初步建立了花生胚小叶体细胞植株再生体系。[结果]在预培养取材条件下,预培养6d的四粒红外植体在2号培养基(MS+3mg/L6-BA+1mg/LNAA)上分化得最好,每愈伤组织再生芽2.34个,预培养5d的改良海花外植体在1号培养基(MS+5mg/L6-BA+3mg/LNAA)上分化最佳,每愈伤组织再生芽2.08个。直接取材条件下,改良海花在1号培养基上每愈伤组织出芽数为6个,而四粒红为3个。直接取材在诱导愈伤组织及器官分化方面都好于预培养取材。[结论]直接取材条件下,不同培养基对诱导愈伤组织及芽分化的作用差异较大,而基因型在诱导愈伤组织上的作用有显著差异,在出芽率上的作用差异并不显著。
[Objective] The plant regeneration system of somatic cell of peanut embryonic leaflet was studied to provide the experimental basis for genetic transformation of exogenous gene by using embryonic leaflet. [Method] With the embryonic leaflets of peanut varieties Silihong and Gailianghaihua as explants and two explant sampling modes （pre-culture and direct explants） as culture background, the relationship between culture factors （genotype, medium, etc.） and dedifferentiation, redifferentiation was discussed systemically under different sampling modes and the plant regeneration system of somatic cell of peanut embryonic leaflet was established preliminarily. [Result] Under the pre-culture explant condition, the explants of Silihong pre-cultured for 6 d dedifferentiated best on No. 2 medium （MS ＋3 mg/L 6-BA ＋ 1 mg/L NAA）, differentiating 2.34 regeneration buds per callus; the explants of Gailianghaihua pre-cultured for 5 days dedifferentiated best on No 1 medium （MS ＋5 mg/L 6-BA＋NAA 3 mg/L）, differentiating 2.08 regeneration buds per callus. Under the direct explant conditions, Gailianghaihua could differentiate 6 regeneration buds per callus and Silihong could differentiate 3 regeneration buds on No 1 medium. The culture effect of direct explants was better than that of pre-cultured explants on callus induction and organ differentiation. [Conclusion] Under the direct explant conditions, the influence of different media had significant difference on callus induction and bud differentiation, while the influence of genotypes had significant difference on callus induction and had no significant difference on sprouting rate
出处
《安徽农业科学》
CAS
北大核心
2007年第26期8135-8137,共3页
Journal of Anhui Agricultural Sciences
关键词
花生
胚小叶
植株再生
Peanut
Embryonic leaflet
Plant regeneration
