摘要
重组肿瘤坏死因子对ＳＷ－４８０结肠癌细胞杀伤作用低敏感，但对细胞克隆生长的抑制作用很经。将ＴＮＦ与５－Ｆｕ联合应用，单用ＴＮＦ９４ｕ／ｍｌ深度对ＳＷ－４８０只有克隆生长抑制作用其杀伤率不到２％，５－Ｆｕ１２．５ｍｇ／Ｌ浓度单用杀伤率只８．６％，但合有香ＴＮＦ杀伤率提高１２７０倍，５－Ｆｕ刹伤率提高１５倍，ＭＭＣ０．６２５ｍｇ／Ｌ浓度单用杀伤率２３．８％，单用ＴＮＦ１．
rhTNF's killing effect on human colon carcinoma cell line SW-480 is low sensitive,whereas itscolony inhibiting effect is strong. The administration of rhTNF with concentration of 94 U/ml had an inhibiting effect on SW-480 with killing rate less than 2%. The administration of 5-Fu at 12. 5 μg/ml showed a killing rate of 8.6%. However, the effect of rhRNF rose 1270 times and 5-Fu 15 times when the two were used concomitantly. Furthermore, when used alone at 0.625 mg/mland 1.2×104U/ml,MMC's and rhTNF's killing rate was 23.8% and less than 5% , respectively. The evidents suggested that rhTNF plus 5-Fu or MMC produced obvious synergic effects in vitro. For clinical application, these synergic effects could reduce the dose of drugs, the toxic effects and the cost of therapy.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1997年第3期139-140,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目
关键词
肿瘤坏死因子
结肠肿瘤
recombinant human tumor necrosis factor
human colon carcinoma cell
inhibition
cellular toxic
DNA syntheses
synergic effect
