摘要
目的:通过体外实验探讨紫杉醇(paclitaxel,TAX)对人胰腺癌细胞株Bx-PC-3的增殖抑制作用。方法:人胰腺癌细胞株BxPC-3经过不同浓度的TAX作用不同时间后,在倒置显微镜下观察细胞形态学改变;用甲基噻唑基四唑试验(MTT)检测细胞抑制率;用流式细胞仪分析细胞周期变化。结果:TAX对人胰腺癌细胞株BxPC-3有明显的生长抑制作用,且呈剂量、时间依赖关系。流式细胞仪分析结果显示,BxPC-3细胞经过1000nmol/LTAX分别作用24和48h后,G2～M期细胞比例分别为(66.39±0.23)%和(48.49±1.47)%,明显高于对照组(8.67±2.32)%和(10.85±2.43)%,t值分别为49.41和26.49,P值均为0.000,提示存在G2～M期阻滞。结论:TAX可使人胰腺癌细胞株BxPC-3的生长受到抑制,BxPC-3细胞经过TAX作用后被阻滞于G2～M期。
OBJECTIVE ： To assess the anti-proliferative effect of paclitaxel on human pancreatic cancer cell line BxPC-3 in vitro. METHODS： The human pancrecatic cancer cell line BxPC-3 was treated with paclitaxel of different concentrations for different time. The changes of cell morphology were observed under an inverted light microscope. The inhibition rates of cells were detected through MTT assay. The changes of cell cycle were analysed through flow cytometry. RESULTS.. The growth inhibhion effect of paclitaxel was observed on human pancreatic cancer cell line Bx- PC-3, and the growth inhibition effect was time and dosage dependent. The result of flow cytometry showed that cells in G2 -M phase were increased after exposure to 1 000 nmol/L paclitaxel for 24 h and 48 h, the proportions of cells in G2 - M phase were （66.39±0.23）% and （48. 49± 1.47）%, and those in control group were （8.67±2.32）% and （10.85±2.43）%, t was 49.41 and 26.49, P was 0. 000 and 0. 000, indicating that the cells were blocked in G2-M phase. CONCLUSION.. Growth inhibhion effect is of paclitaxel observed on human pancreatic cancer cell line BxPC-3, and the cells exposed to paclhaxel are blocked in G2-M phase.
出处
《中华肿瘤防治杂志》
CAS
2007年第22期1694-1697,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
胰腺肿瘤/病理学
增殖抑制
流式细胞术
抗肿瘤药
pancreatic neoplasms/pathology
proliferative inhibition
flow cytometry
antineoplastic agents
